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目的检测T细胞免疫球蛋白域黏蛋白结构域分子3(T cell immunoglobulin and mucin domain protein 3,Tim-3)在晚期血吸虫病患者外周血CD4+T细胞表面的表达,并探讨其与肝功能损伤指标的关系。方法以28例晚期血吸虫病患者作为研究对象,同时选择20例慢性血吸虫病患者和30例健康人群作为对照,采用流式细胞术检测CD4+T细胞Tim-3表达水平,用ELISA法检测血清干扰素-γ(IFN-γ)和白细胞介素-4(IL-4)水平,以日立7600型生化分析仪检测肝功能指标丙氨酸氨基转移酶(ALT)、γ-谷氨酰转移酶(γ-GT)和总胆红素(TBIL)浓度。结果晚期血吸虫病、慢性血吸虫病患者以及健康人群外周血CD4+T细胞表面Tim-3表达水平差异具有统计学意义(F=4.578,P<0.05),晚期血吸虫病患者外周血CD4+T细胞Tim-3表达水平为(8.33±2.28)%,显著高于健康对照人群的(6.57±1.99)%(t=3.015,P<0.01)。Spearman相关分析显示,晚期血吸虫病患者外周血CD4+T细胞Tim-3表达水平与患者血清ALT(rs=0.746,P<0.01)、γ-GT(rs=0.656,P<0.01)、IL-4水平(rs=0.672,P<0.01)呈正相关,与IFN-γ水平(rs=-0.404,P<0.05)呈负相关。结论晚期血吸虫病患者外周血CD4+T细胞Tim-3表达上调,其可能通过调节CD4+T细胞功能参与晚期血吸虫病肝损伤过程。
Objective To detect the expression of T cell immunoglobulin and mucin domain protein 3 (Tim-3) on the surface of CD4 + T cells in peripheral blood of patients with advanced schistosomiasis, and to explore its relationship with liver injury The relationship between indicators. Methods Twenty-eight patients with advanced schistosomiasis were enrolled in this study. Twenty patients with chronic schistosomiasis and 30 healthy controls were selected as controls. The expression of Tim-3 in CD4 + T cells was detected by flow cytometry. Serum interference Γ-glutamyl transferase (ALT) and γ-glutamyl transferase (IL-4) were measured with Hitachi 7600 biochemical analyzer. γ-GT) and total bilirubin (TBIL) concentrations. Results The expression of Tim-3 on peripheral blood CD4 + T cells in patients with advanced schistosomiasis and chronic schistosomiasis was significantly different from that in healthy controls (F = 4.578, P <0.05) -3 (8.33 ± 2.28)%, which was significantly higher than that of healthy controls (6.57 ± 1.99)% (t = 3.015, P <0.01). Spearman correlation analysis showed that the expression level of Tim-3 in CD4 + T cells in patients with advanced schistosomiasis was significantly correlated with serum ALT (rs = 0.746, P <0.01), γ-GT (Rs = 0.672, P <0.01), but negatively correlated with the level of IFN-γ (rs = -0.404, P <0.05). Conclusion The expression of Tim-3 in CD4 + T cells in peripheral blood of patients with advanced schistosomiasis is up-regulated, which may be involved in the process of liver injury in advanced schistosomiasis by regulating the function of CD4 + T cells.