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OBJECTIVE To investigate the correlation betweenpolymorphism of the 5’-untranslated region (5’-UTR) ofthymidylate synthase genes,as well as the lifestyle,and thesusceptibility of gastric carcinoma.METHODS A case-control study,with 60 cases of gastriccarcinoma and 170 cases of general risk population-based controlsfrom Nantong,Jiangsu province,China,was conducted.Theepidemiological data,such as living habits of the cancer patients,were collected.DNA of peripheral blood leukocytes was obtainedfrom all of the subjects.The TS 5’-UTR tandem repeat genotypewas detected using polymerase chain reaction (PCR).RESULTS There were three TS 5’-UTR genotypes in the groupof gastric cancer cases (2R/2R,2R/3R and 3R/3R) and six TS5’-UTR genotypes in the group of the controls (2R/2R,2R/3R,3R/3R,2R/4R,2R/5R and 3R/4R).The genotypic frequencies wererespectively 5.0%,43.3% and 51.7% in the gastric cancer group.Compared with the parameters in the control group,i.e.,4.7%,31.7%,60.6%,1.2%,1.2% and 0.6%.There were no significantdifferences between the two groups.Compared with the 3R/3R-genotype individuals who where non-smokers,drank alcoholtwice or less each week,drank tea and did not intake pickled food(PF),the risk of gastric cancer significantly went up in the 2R/2Ror 2R/3R-genotype people who had habits of smoking,drinkingalcohol more than twice each week,no tea drinking but withfrequent intake of PF.The adjusted ORs were as follows,3.79 (95%CI:2.45～8.64),3.41 (95% CI:1.21～8.47),5.99 (95% CI:3.01～14.7),and 3.61 (95% CI:1.81～8.78).CONCLUSION There is an obvious correlation between thepolymorphisms of TS 5’-UTR genotypes and the lifestyle ofindividuals in the development of gastric carcinoma.
OBJECTIVE To investigate the correlation between polymorphism of the 5’-untranslated region (5’-UTR) of thymidylate synthase genes, as well as the lifestyle, and the susceptibility of gastric carcinoma. METHODS A case-control study, with 60 cases of gastriccarcinoma and 170 cases of general risk population-based controlsfrom Nantong, Jiangsu province, China, was conducted.Theepidemiological data, such as living habits of the cancer patients, were collected. DNA of peripheral blood leukocytes was obtainedfrom all of the subjects.The TS 5’-UTR tandem repeat genotype was detected using polymerase chain reaction (PCR) .RESULTS There were three TS 5’-UTR genotypes in the group of gastric cancer cases (2R / 2R, 2R / 3R and 3R / 3R) and six TS5’-UTR genotypes in the The genotypic frequencies were potentially 5.0%, 43.3% and 51.7% in the gastric cancer group. Compared with (2R / 2R, 2R / 3R, 3R / 3R, 2R / 4R, 2R / 5R and 3R / 4R) the parameters in the control group, ie, 4.7%, 31.7%, 60.6%, 1.2%, 1.2% and 0.6% .There were no significant differences between the two groups. Compared with the 3R / 3R-genotype individuals who where non-smokers, drank alcohol twice or less each week, drank tea and did not intake pickled food (PF), the risk of gastric cancer significantly went up in the 2R / 2Ror 2R / 3R-genotype people who had habits of smoking, drinking alcohol more than twice each week, no tea drinking but with subsequent intake of PF. 3.79 (95% CI: 2.45-8.64) , 3.41 (95% CI: 1.21-8.47), 5.99 (95% CI: 3.01-14.7), and 3.61 (95% CI: 1.81-8.78) .CONCLUSION There is an obvious correlation between the polymorphisms of TS 5’-UTR genotypes and the lifestyle of individuals in the development of gastric carcinoma