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目的:以牛血清白蛋白(BSA)作为模型药物,制备壳聚糖/有机累托石复合物微球,建立一种安全有效的药物控释传递系统。方法:壳聚糖(CS)/有机累托石(OREC)和海藻酸钠,按照不同的混合比例交联,在Ca2+水溶液中包裹BSA而形成壳核结构的微球。采用傅立叶红外光谱(FTIR)、动态光散射(DLS)、原子力显微镜(AFM)、X-衍射(XRD)、扫描电镜(SEM)和透射电镜(TEM)观察研究微球的形态、CS和OREC的插层结构、BSA的包封率和控释效果。结果:口光学显微镜和扫描电镜观察显示,形成了壳核结构的微球。傅里叶变换光谱和X-射线能量分散显示,OREC存在于微球中。小角X-射线衍射证实,CS链成功的插入OREC插层中。BSA的包封率和控释检测结果显示,与纯的CS/ALG形成的微球相比较,CO复合物所形成的微球药物释放率明显提高。结论:OREC-HTCC纳米粒子是良好的蛋白药物载体,具有包封率高、缓释效果好等优点,为CS-OREC作为潜在的药物给药系统的进一步应用提供科学依据。
OBJECTIVE: To prepare chitosan / organic rectorite microspheres with bovine serum albumin (BSA) as a model drug and establish a safe and effective drug delivery system. METHODS: Chitosan (CS) / organic rectorite (OREC) and sodium alginate were cross-linked at different mixing ratios to encapsulate BSA in Ca2 + aqueous solution to form microspheres with shell-like structure. The morphologies of the microspheres were observed by Fourier transform infrared spectroscopy (FTIR), dynamic light scattering (DLS), atomic force microscopy (AFM), X-ray diffraction (XRD), scanning electron microscopy (SEM) and transmission electron microscopy (TEM) Intercalation structure, BSA encapsulation efficiency and controlled release effect. Results: Oral light microscopy and scanning electron microscopy revealed the formation of microspheres with putamen structure. Fourier transform spectroscopy and X-ray energy dispersal showed that OREC was present in the microspheres. Small angle X-ray diffraction confirmed that the CS chain was successfully inserted into the OREC intercalation. The encapsulation efficiency and controlled release test results of BSA showed that the release rate of microsphere drug formed by CO complex was significantly higher than that of pure CS / ALG formed microspheres. CONCLUSION: OREC-HTCC nanoparticle is a good carrier for protein drug, which has the advantages of high encapsulation efficiency and good sustained-release effect, and provides a scientific basis for the further application of CS-OREC as a potential drug delivery system.