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目的:探讨人类胎盘提取液对高脂饮食性脂肪肝大鼠血清中TNF-α、IL-6的影响。方法:34只W istar大鼠,雄性,分为正常组、模型组、胎盘提取液高剂量组和低剂量组,予既定试剂4周后,取肝组织行HE染色观察组织学改变,用放免法检测各组大鼠血清中TNF-α、IL-6的含量。结果:模型组血清中TNF-α、IL-6的含量较正常组显著升高(P<0.05或P<0.01),胎盘提取液高、低剂量组较模型组显著降低(P<0.05或P<0.01)。组织学观察模型组均出现了中度以上大泡性肝脂肪变性,胎盘提取液低剂量组较模型组有一定程度的改善,胎盘提取液高剂量组脂肪浸润状态较模型组有明显好转。结论:TNF-α、IL-6参与脂肪性肝病的发生。人类胎盘提取液对高脂饮食性脂肪肝具有显著疗效,作用机制可能与其显著下调血清中TNF-α、IL-6水平有关。
Objective: To investigate the effect of human placental extract on serum TNF-α and IL-6 in rats with high fat dietary fatty liver. Methods: Thirty-four Wistar rats were divided into normal group, model group, high-dose group and low-dose placenta extract group. Four weeks later, liver tissues were taken for histological changes and histopathological changes were observed. The serum levels of TNF-α and IL-6 were measured in each group. Results: The levels of TNF-α and IL-6 in the serum of the model group were significantly higher than those in the normal group (P<0.05 or P<0.01). The high and low doses of the placenta extract were significantly lower than the model group (P<0.05 or P). <0.01). Histological observation model group showed moderate to large bullous steatosis, and the placenta extract low-dose group had a certain degree of improvement compared with the model group. The high-dose placenta extract high-dose group had significantly better fat infiltration than the model group. Conclusion: TNF-α and IL-6 are involved in the occurrence of fatty liver disease. Human placenta extract has a significant effect on high-fat dietary fatty liver, and its mechanism may be related to its significant down-regulation of TNF-α and IL-6 levels in serum.