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目的研究参附注射液(简称参附)对大鼠肠缺血再灌注期间黏膜上皮细胞凋亡的影响,并探讨其可能机制。方法采用大鼠肠缺血再灌注模型。24只大鼠随机分为对照组(C组)、缺血再灌注组(I/R组)和参附治疗组(SF组),SF组于阻断前30min静注SF液2mL/100g。再灌注2h检测回肠黏膜上皮细胞Cas-pase-3,Bcl-2蛋白的表达及细胞凋亡;同时观察小肠黏膜病理形态学改变。结果凋亡细胞检测显示:I/R组凋亡细胞显著增多,SF组凋亡细胞数较I/R组显著减少而多于C组(均P<0.05);Caspase-3,Bcl-2蛋白的表达:Caspase-3表达I/R组显著多于SF组和C组(P<0.01),SF组与C组无明显差异;Bcl-2表达SF组显著高于I/R组(P<0.05),后者显著低于C组(P<0.05);SF组小肠黏膜病理损伤程度明显减轻I/R组(P<0.01)。结论参附注射液通过抑制Caspase-3表达和促进Bcl-2基因表达减少缺血再灌注期间肠黏膜上皮细胞的凋亡,从而减轻肠黏膜缺血再灌注损伤。
Objective To study the effect of Shenfu injection (Shenfu) on mucosal epithelial cell apoptosis during intestinal ischemia-reperfusion in rats, and to explore its possible mechanism. Methods Rat intestinal ischemia-reperfusion model was used. Twenty-four rats were randomly divided into control group (C group), ischemia-reperfusion group (I/R group) and Shenfu treatment group (SF group). SF group was intravenously injected with 2mL/100g SF solution 30 minutes before blocking. The expression of Cas-pase-3 and Bcl-2 protein in ileal mucosa epithelial cells and cell apoptosis were detected at 2h after reperfusion. Pathological changes of intestinal mucosa were also observed. Results Apoptotic cells showed a significant increase in apoptotic cells in the I/R group. The number of apoptotic cells in the SF group was significantly lower than that in the I/R group (all P<0.05); Caspase-3 and Bcl-2 proteins The expression of Caspase-3 in the I/R group was significantly more than that in the SF group and the C group (P<0.01). There was no significant difference between the SF group and the C group. The expression of the Bcl-2 in the SF group was significantly higher than that in the I/R group (P< 0.05), the latter was significantly lower than the C group (P <0.05); SF group pathological lesions of the small intestine mucosa significantly reduced I / R group (P <0.01). Conclusion Shenfu injection inhibits the expression of Caspase-3 and promotes the expression of Bcl-2 gene to reduce the apoptosis of intestinal mucosal epithelial cells during ischemia-reperfusion, thus reducing the intestinal mucosal ischemia-reperfusion injury.