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目的探讨高效氯氰菊酯对家蝇乙酰胆碱酯酶(AChE)、过氧化氢酶(CAT)、谷胱甘肽硫转移酶(GST)3种常见酶活性变化的作用。方法在抗性培育过程中用试剂盒测定家蝇3种酶的活性,分析其变化趋势。结果在抗性培育过程中,在杀虫剂剂量为0.1 mg/ml作用下,3种酶活性均有不同程度的增加。在选育到第18代时,AChE活性由敏感体系的0.064 U/mgprot增至11.693 U/mgprot;在选育到第16代时,CAT活性由敏感体系的1.062 U/mgprot增至19.331 U/mgprot;在选育到第18代时,GST活性由最初的171.77 U/mgprot增至611.814 U/mgprot。结论乙酰胆碱酯酶、过氧化氢酶、谷胱甘肽硫转移酶3种酶可能均与抗药性的产生有关。
Objective To investigate the effects of beta-cypermethrin on the changes of three common enzyme activities of acetylcholinesterase (AChE), catalase (CAT) and glutathione S-transferase (GST) in Musca domestica. Methods The activity of three kinds of enzymes in housefly was determined by kit during the process of resistance cultivation, and the change trend was analyzed. Results During the process of resistance cultivation, the activities of all three enzymes increased to different extents under the pesticide dosage of 0.1 mg / ml. At the 18th generation, the AChE activity increased from 0.064 U / mgprot to 11.693 U / mgprot in the sensitive system. From the 16th generation to the 16th generation, CAT activity increased from 1.062 U / mgprot in the sensitive system to 19.331 U / mgprot; At the 18th generation, GST activity increased from the initial 171.77 U / mgprot to 611.814 U / mgprot. Conclusion The three enzymes of acetylcholinesterase, catalase and glutathione S-transferase may be involved in the development of drug resistance.