Construction of the recombinant vector carrying herpes simplex virus thymidine kinase and cytokine g

• 作 者: 蓟光辉 邹敬之 渠乐 岳瑛 蒯建科
• 机 构: DepartmentofOdontology,DepartmentofOdontology,DepartmentofOdontology,DepartmentofOdontology,Departme
• 出 处: Journal of Medical Colleges of PLA
• 发表日期: 2004年3期
• 关键词: 激酶致活酶 单纯性疱疹病毒 胸苷激酶 胞质分裂基因 基因表达 Tca8113细胞系统 PCR herpes simplex virus thymidine ki

Objective: To construct expression vector containing fusion genes of herpes simplex virus thymidine kinase(Hsv-tk), Interleukin-2(IL-2) with internal ribosome entry sites(IRES), and to assess their expression in cell lineTca8113. Methods: IL-2 cDNA was ob