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目的探讨雌激素(17β-estradiol,17β-E2)对B段紫外线(UVB)辐射引起皮肤损伤的保护作用及其保护机制。方法体外培养人永生化角质形成细胞(HaCaT),并将其随机分为4组:对照组、UVB组、E2组和E2+UVB组,分别给予不同的处理,经UVB照射后6 h,收集各组细胞上清液,应用酶联免疫吸附试验(ELISA)方法检测UVB照射后6 h各组细胞上清液中白细胞介素10(IL-10)、白细胞介素6(IL-6)和肿瘤坏死因子α(TNF-α)分泌量的变化;UVB照射后12 h,收集各组细胞,采用单细胞凝胶电泳技术检测各组细胞的DNA损伤程度。结果与对照组相比较,UVB组IL-10、IL-6和TNF-α的含量显著升高(P<0.05);经E2预处理后,E2+UVB组细胞上清液中IL-10、IL-6和TNF-α的含量均显著降低(P<0.05)。单细胞凝胶电泳检测结果显示,UVB组和E2+UVB组均出现彗星样拖尾,应用CASP和SPSS软件分析统计后得出,UVB组细胞尾部DNA百分含量、尾长和尾矩与对照组相比具有统计学意义;经E2预处理后,UVB对细胞损伤程度明显降低,具有统计学意义(P<0.05)。结论雌激素可降低UVB引起的IL-10、IL-6和TNF-α的产生,并能对抗UVB照射对细胞DNA的损伤,从而减轻紫外线辐射引起的皮肤损伤。
Objective To investigate the protective effect of 17β-estradiol (17β-E2) on skin lesions induced by ultraviolet B (UVB) radiation and its protective mechanism. Methods Human immortalized keratinocytes (HaCaT) were cultured in vitro and randomly divided into 4 groups: control group, UVB group, E2 group and E2 + UVB group, which were given different treatments. After 6 hours of UVB irradiation, The supernatant of each group was tested for the levels of IL-10, IL-6 and IL-6 in supernatant of each group by enzyme-linked immunosorbent assay (ELISA) Tumor necrosis factor α (TNF-α) secretion changes; 12 h after UVB irradiation, each group of cells were collected, single cell gel electrophoresis was used to detect DNA damage in each group of cells. Results Compared with control group, the levels of IL-10, IL-6 and TNF-α in UVB group were significantly increased (P <0.05). After E2 pretreatment, the levels of IL-10, The levels of IL-6 and TNF-α were significantly decreased (P <0.05). The result of single cell gel electrophoresis showed that comet-like tailings occurred in both UVB group and E2 + UVB group. The percentage of tail DNA, tail length and tail moment of UVB group were compared with those of control Group compared with the statistically significant; pretreated with E2, UVB on the cell damage significantly reduced, with statistical significance (P <0.05). Conclusion Estrogen can reduce the production of IL-10, IL-6 and TNF-α induced by UVB and protect against DNA damage caused by UVB irradiation and thus reduce the skin damage caused by ultraviolet radiation.