论文部分内容阅读
目的 :观察柔红霉素对兔晶状体上皮细胞和眼前节组织的影响。方法 :2 0只家兔随机分为两组 ,分别行EC CE +IOL植入术。实验组截囊前于前囊下注入 8μg/ml柔红霉素 0 .2ml,对照组用同量乳酸林格氏液 ,作用 5min。术后观察眼前节变化及眼压。于术后第 7、第 14、第 2 8天共处死18只家兔 ,行后囊膜、角膜、虹膜、睫状体行光学显微镜观察。两组各有 1只家兔于术后第 2 8天处死 ,行后囊膜透射电镜观察。结果 :①光镜下 ,对照组术后各组后囊晶状体上皮细胞增殖 ,数量较多。实验组术后 7天时其与对照组相同。第 14天 ,实验组后囊可见细胞增生 ,细胞核退变 ,细胞密度明显降低。 2 8天时细胞退变 ,密度进一步降低。②电镜下 ,对照组细胞结构完整。实验组细胞变形 ,内部结构不清 ,核质溶解。③两组眼前段组织显示一般手术反应 ,其严重程度、持续时间相同。裂隙灯下术后第 2 8天后囊膜混浊实验组明显轻于对照组。结论 :8μg/ml柔红霉素于截囊前注入晶状体前囊下 ,作用 5min ,可破坏残留的晶状体上皮细胞的微结构 ,短期内抑制家兔晶状体后囊上皮细胞增殖 ,减轻后囊混浊。同期内未观察到对正常眼组织的毒副作用。该药作为抑制晶状体上皮细胞增殖临床用药 ,具有乐观的前景
Objective: To observe the effect of daunorubicin on the lens epithelial cells and anterior segment of rabbits. Methods: Twenty rabbits were randomly divided into two groups and received ECCE + IOL implantation respectively. The experimental group before injection into the anterior capsule 8μg / ml daunorubicin 0.2ml, the control group with the same amount of lactated Ringer’s solution for 5min. Postoperative anterior segment changes and intraocular pressure were observed. Eighteen rabbits were sacrificed on the 7th, 14th and 28th day after operation. The posterior capsule, cornea, iris and ciliary body were observed with optical microscope. One rabbit in each group was sacrificed on the 28th day after operation, and the posterior capsule was observed by transmission electron microscopy. Results: ① Under light microscope, the number of proliferative and ophthalmic lens epithelial cells in control group was higher than that in control group. Experimental group 7 days after the same as the control group. On the 14th day, the posterior capsule of the experimental group showed hyperplasia of cells, degeneration of nucleus and marked decrease of cell density. 2 8 days degeneration, further reduce the density. ② electron microscope, the control group cell structure is complete. Experimental group of cells deformed, the internal structure is unclear, dissolved nuclear. ③ the two groups of anterior segment of the organization showed a general surgical response, the severity, the same duration. Slit lamp after 28 days postoperative capsule opacity experimental group was significantly lighter than the control group. CONCLUSION: The injection of 8μg / ml daunorubicin into the anterior capsule of the lens before the capsulotomy for 5min can destroy the microstructure of the residual lens epithelial cells and inhibit the proliferation of posterior capsule lens epithelial cells and the posterior capsule opacification in a short term. The same period did not observe the side effects of normal eye tissue. The drug has an optimistic outlook as a clinical drug for inhibiting the proliferation of lens epithelial cells