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由EB病毒转化的淋巴母细胞样细胞系(LcL)可产生免疫球蛋白已得到普遍确认。1980年Tsuchiya等采用破伤风和白喉类毒素免疫儿童和成人后,在EB病毒转化的LcL培养悬液中可产生两者的相应抗体。对半抗原、4-羟基3,5二硝基苯乙酸具有高滴度抗体供者的淋巴细胞建立的LcL,体外培养可用来制备特异性抗体。1981年Wands等从免疫动物的淋巴细胞与小鼠骨髓瘤细胞系融合获得的杂交体细胞可产生大量的特异小鼠抗体。
The production of immunoglobulins by the Epstein-Barr virus-transformed lymphoblastoid cell line (LcL) has been generally recognized. In 1980, Tsuchiya et al. Used tetanus and diphtheria toxoid-immunized children and adults to generate the corresponding antibodies in EBV-transformed LcL culture suspensions. For LcL, a hapten, 4-hydroxy 3,5-dinitrophenylacetic acid with high titer antibody donor lymphocytes, in vitro culture can be used to prepare specific antibodies. In 1981, Wands et al. Generated a large number of specific mouse antibodies from hybrid cells obtained by immunizing the lymphocytes of immunized animals with the mouse myeloma cell line.