论文部分内容阅读
目的探讨脱细胞真皮基质(ADM)以戊二醛为交联剂复合羧甲基壳聚糖(CMC)后的抗菌性能。方法实验采用2×3析因设计,根据样本状态(湿润、干燥)和分组(复合样片组、单纯样片组、空白对照组)设计实验。应用烧瓶振荡法,利用优化的实验条件,在转速200 r/min、培养温度25℃、作用时间1 h的条件下,对复合CMC的ADM进行抗菌性能检测。通过菌落平板计数获得实验前(0 h)、实验后(1 h)的菌落数,计算抑菌率。两组实验中细菌在振摇前后的菌落数差值均符合实验限定标准(差值变化不超过10%)。结果湿润状态下,复合样片组对金黄色葡萄球菌的抑菌率为61.17%,对白色念珠菌的抑菌率为39.79%;干燥状态下,复合样片组对金黄色葡萄球菌的抑菌率为0%,对白色念珠菌的抑菌率为15.17%。湿润与干燥两个因素比较,差异有统计学意义(P<0.001)。结论复合CMC的ADM这种交联复合物在湿润状态下有一定的抗菌性,干燥状态下不具备抗菌性能,交联效率较低,需进一步改良制备工艺。
Objective To investigate the antimicrobial activity of ADM with glutaraldehyde as cross-linking agent and carboxymethyl chitosan (CMC). Methods The experiment was designed by 2 × 3 factorial design, and the experiment was designed according to the state of the sample (wetting and drying) and grouping (compound swatch, simple swatch and blank control). By using flask shaking method, the antibacterial property of ADM with CMC was tested under the optimized experimental conditions at the rotational speed of 200 r / min, the incubation temperature of 25 ℃ and the action time of 1 h. The number of colonies before experiment (0 h) and after experiment (1 h) was obtained by colony plate counting, and the antibacterial rate was calculated. Bacteria in both groups before and after shaking the number of colonies are in line with experimentally defined limits (the difference does not exceed 10%). Results Under the wet condition, the antibacterial rate of the composite sample group to Staphylococcus aureus was 61.17% and the antibacterial activity to Candida albicans was 39.79%. The antibacterial rate of the composite sample group against Staphylococcus aureus was 0%, Candida albicans inhibition rate of 15.17%. Wet and dry two factors, the difference was statistically significant (P <0.001). CONCLUSION: The crosslinked composite of ADM with CMC has some antibacterial properties under wet condition, no antibacterial property under dry condition, and low crosslinking efficiency, so the preparation process needs to be further improved.