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目的:探讨低频磁场对大鼠心肌微血管内皮细胞(CMECs)增殖和缝隙连接蛋白43(Cx43)表达的影响。方法:设不同照射强度为(08.mT组,1.4mT组,1.8mT组)为照射组。不加磁场干预为对照组。照射条件;磁场频率为15Hz,强度分别为0.8mT、1.4mT、1.8mT,照射时间为4 h/d,连续照射7d。应用MTT法检测CMECs增殖,采用Western blot检测Cx43蛋白表达。结果:生长曲线结果显示,磁场能够促进CMECs增殖。1.4mT组照射第2d后CMECs生长速度加快,在第3d开始进入对数生长期,在第4d生长最为旺盛之后进入生长平台期,第2~7d与对照组比较有显著性差异(P<0.05),而且细胞生长曲线明显前移并且峰值增高。1.4mT组、1.8mT组CMECs增殖与对照组比较显著升高(P>0.05)。磁场照射后Cx43表达明显上调,1.4mT组、1.8mT组Cx43蛋白的表达均明显上升,与对照组比较差异有统计学意义(P<0.05),0.8mT组cx43蛋白的表达与对照组比较无显著性差异(P>0.05)。而Cx43蛋白的表达1.4 mT组和1.8mT组之间差异无统计学意义(P>0.05)。结论:低频脉冲磁场能促进CMECs增殖与增强细胞活力,上调Cx43表达,其在分子水平上的可能作用机制表现为对Cx43的有效调控。
Objective: To investigate the effects of low frequency magnetic field on the proliferation of rat cardiac microvascular endothelial cells (CMECs) and the expression of connexin 43 (Cx43). Methods: The different irradiation intensity (08.mT group, 1.4mT group, 1.8mT group) for the irradiation group. Without magnetic field intervention for the control group. Irradiation conditions; magnetic field frequency of 15Hz, the intensity of 0.8mT, 1.4mT, 1.8mT, irradiation time of 4 h / d, continuous irradiation 7d. The proliferation of CMECs was detected by MTT assay and the expression of Cx43 protein was detected by Western blot. Results: The growth curve results show that magnetic field can promote the proliferation of CMECs. The growth of CMECs was accelerated on the 2nd day after irradiation in the 1.4mT group, and entered into the logarithmic growth phase on the 3rd day after entering the growth plateau stage after the most vigorous growth on the 4th day. There was a significant difference between the 2nd and 7th days when compared with the control group (P <0.05 ), And the cell growth curve significantly advanced and the peak increased. The proliferation of CMECs in 1.4mT group and 1.8mT group was significantly higher than that in control group (P> 0.05). The expression of Cx43 in the 1.4mT group and 1.8mT group increased obviously after irradiation with magnetic field (P <0.05), the expression of cx43 protein in 0.8mT group was lower than that in the control group Significant difference (P> 0.05). The expression of Cx43 protein between 1.4 mT group and 1.8 mT group had no significant difference (P> 0.05). CONCLUSION: Low-frequency pulsed magnetic field can promote the proliferation and enhance the cell viability of CMECs, and up-regulate the expression of Cx43. The possible mechanism of its action at molecular level is the effective regulation of Cx43.