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目的 探讨大鼠肾缺血再灌注损伤不同时间c-fos、bcl-2、ICAM -1蛋白的表达及地塞米松对其影响。方法 用免疫组化法检测大鼠急性肾缺血再灌注不同时间内及地塞米松干预后c-fos、bcl-2、ICAM -1蛋白表达的分布及强度变化。结果 c-fos蛋白分布于近曲小管、远曲小管、集合管上皮细胞的细胞核、细胞浆内 ,再灌注后 1h表达明显增强 ,3h达高峰 ,6h锐减。bcl-2蛋白主要分布于近曲小管上皮细胞的细胞浆 ,再灌注后 1h表达明显增强 ,6h达高峰 ,2 4h仍有较强表达。ICAM -1蛋白分布在肾血管、肾小管等部位 ,其中以肾血管为著 ,其表达增强于再灌注后 1h ,直到 2 4h仍有增高趋势。地塞米松干预后c-fos、ICAM -1蛋白表达明显下降 (P <0 0 1 )。bcl-2表达明显增高 (P <0 0 1 )。结论 地塞米松对肾缺血再灌注损伤有较好的保护作用
Objective To investigate the expression of c-fos, bcl-2 and ICAM-1 at different time points after renal ischemia-reperfusion injury and the effect of dexamethasone on it. Methods Immunohistochemistry was used to detect the expression of c-fos, bcl-2 and ICAM-1 protein in acute renal ischemia-reperfusion injury and dexamethasone intervention in rats. Results The c-fos protein was distributed in the proximal convoluted tubule, distal convoluted tubule and the collecting duct epithelial cells in the nucleus and cytoplasm. The expression of c-fos protein was significantly increased at 1 hour and peaked at 3 hours, sharply decreased at 6 hours. The bcl-2 protein was mainly distributed in the cytoplasm of proximal tubule epithelial cells. The expression of bcl-2 protein was significantly increased at 1 hour after reperfusion, reaching the peak at 6 hours and remained strong at 24 hours. ICAM-1 protein distributed in the renal blood vessels, tubules and other parts, of which the renal vessels, the expression increased 1h after reperfusion, until 24h still increased. Dexamethasone intervention c-fos, ICAM -1 protein expression decreased significantly (P lt; 0 0 1). bcl-2 expression was significantly increased (P <0.01). Conclusion Dexamethasone has a good protective effect on renal ischemia-reperfusion injury