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目的研究湖南芹菜籽挥发油的化学成分,为其质量评价提供依据。方法同时蒸馏萃取法提取湖南芹菜籽挥发油,用GC/MS法分析挥发油成分;通过Nist11质谱库进行检索,利用计算的保留指数与ESO精油数据库中的保留指数进行比对,对其成分进行定性;采用色谱峰面积归一化法计算得出各成分的相对百分含量。结果从湖南芹菜籽挥发油中鉴定出49种成分,挥发油中各种成分占总峰面积的83.830%。主要成分为柠檬烯(44.679%),β-芹子烯(14.661%),α-芹子烯(5.939%),3-正丁基苯酞(4.709%),β-蒎烯(2.525%),γ-芹子烯(2.073%),月桂烯(1.215%),苯戊酮(1.172%),β-桉叶油醇(0.827%),α-蒎烯(0.543%),石竹烯氧化物(0.540%)等。结论采用保留指数鉴别同系物及同分异构体,提高了湖南芹菜籽挥发油成分定性准确性,为湖南芹菜籽产品开发和应用提供参考。
Objective To study the chemical constituents of the essential oil of Hunan celery seeds and provide the basis for its quality evaluation. Methods At the same time, the essential oil of celery seed in Hunan was extracted by distillation method. The constituents of volatile oil were analyzed by GC / MS. The chemical constituents were identified by Nist11 mass spectrometry library. The calculated retention index was compared with the retention index of ESO essential oil database. ; Using the peak area normalization method to calculate the relative percentage of each component. Results Forty-nine components were identified from the volatile oil of celery seed in Hunan. The components of the volatile oil accounted for 83.830% of the total peak area. The main components were limonene (44.679%), β-cetoleone (14.661%), α-paeoniflorin (5.939%), 3-n-butylphthalide (4.709% γ-Celery (2.073%), Myrcene (1.215%), Pentanophenone (1.172%), β-Eucalyptol (0.827%), α-Pinene (0.543%), Caryophyllene oxide 0.540%) and the like. Conclusion The use of retention index to identify homologues and isomers can improve the qualitative accuracy of Hunan celery seed oil and provide reference for the development and application of celery seed in Hunan Province.