论文部分内容阅读
目的：观察丙型肝炎病毒（ＨＣＶ）核心蛋白ＤＮＡ疫苗诱导ＢＡＬＢ／ｃ小鼠体液免疫应答的效力，为研制应用于人类的ＨＣＶＤＮＡ疫苗提供实验依据。方法：将全长ＨＣＶ核心蛋白基因插入真核表达质粒载体ｐｃＤＮＡ３．１，构建ＨＣＶ核心蛋白重组表达质粒ｐｃＤＮＡ３．１ＨＣＶｃｏｒｅ，肌注ＢＡＬＢ／ｃ小鼠，以ＥＬＩＳＡ法检测小鼠血清ＨＣＶ抗体。以此重组质粒转染小鼠ＮＩＨ３Ｔ３细胞，以ＨＣＶ抗体阳性小鼠血清为捕获抗体进行Ｗｅｓｔｅｒｎｂｌｏｔ，检测ＤＮＡ疫苗编码的ＨＣＶ核心蛋白的表达。结果：３０％（３／１０）免疫小鼠产生ＨＣＶ核心蛋白抗体，该抗体能检测到重组质粒在ＮＩＨ３Ｔ３细胞表达的ＨＣＶ核心蛋白。结论：ＨＣＶ核心蛋白ＤＮＡ疫苗能诱导ＢＡＬＢ／ｃ小鼠体液免疫应答
Objective: To observe the efficacy of Hepatitis C virus (HCV) core protein DNA vaccine in inducing humoral immune response in BALB / c mice and provide experimental evidence for the development of human HCVDNA vaccine. Methods: The full-length HCV core protein gene was inserted into the eukaryotic expression plasmid vector pcDNA3.1 to construct HCV core protein recombinant plasmid pcDNA3.1 HCVVore, BALB / c mice were intramuscularly injected, and the serum anti-HCV antibodies were detected by ELISA. The recombinant plasmids were transfected into mouse NIH3T3 cells and HCV antibody-positive mouse sera were used as the capture antibody for Western blotting to detect the expression of HCV core protein encoded by the DNA vaccine. RESULTS: Thirty (3/10) immunized mice developed HCV core protein antibodies that detected the HCV core protein expressed by recombinant plasmids in NIH3T3 cells. Conclusion: HCV core DNA vaccine can induce humoral immune response in BALB / c mice