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目的分析中国人群细胞毒性T淋巴细胞相关抗原-4(Cytotoxic T lymphocyte associated antigen-4,CTLA-4)+49位点A>G基因多态性与结直肠癌发生和进展的相关性。方法选取结直肠癌患者248例(疾病组)、结直肠腺瘤患者380例(对照组),采用聚合酶链反应-限制性片段长度多态性(Restriction fragment length polymorphism,RFLP)检测CTLA-4+49位点基因型。结果疾病组CTLA-4+49位点出现GG纯合子或至少出现1个G等位基因的频率显著高于对照组;与携带CTLA-4+49A/A基因型患者相比,携带CTLA-4+49G/G基因型患者发生结直肠癌的危险性为OR=3.684(95%CI:1.670～8.128);携带A等位基因的患者发生远处转移的几率较高,但差异无统计学意义(P>0.05)。结论 CTLA-4+49A>G基因多态性与中国人群结直肠癌易感性具有一定的相关性,但对结直肠癌进展的影响不明显。
Objective To analyze the association between Cytotoxic T lymphocyte associated antigen-4 (CTLA-4) +49 A> G gene polymorphism and the occurrence and progression of colorectal cancer in Chinese population. Methods 248 cases of colorectal cancer (disease group) and 380 cases of colorectal adenoma (control group) were selected. The expression of CTLA-4 was detected by restriction fragment length polymorphism (RFLP) +49 locus genotype. Results The frequency of GG homozygote or at least one G allele in CTLA-4 + 49 locus was significantly higher than that in control group. Compared with patients carrying CTLA-4 + 49A / A genotype, CTLA-4 + The risk of colorectal cancer in patients with +49 G / G genotype was OR = 3.684 (95% CI: 1.670-8.128). Patients with A allele had a higher risk of distant metastasis, but the difference was not statistically significant (P> 0.05). Conclusion The polymorphism of CTLA-4 + 49A> G is associated with the susceptibility of colorectal cancer in Chinese population, but it has no obvious effect on the progression of colorectal cancer.