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[目的]探讨不同浓度的PP333处理对库尔勒香梨叶片组织结构的影响,为库尔勒香梨的栽培调控奠定基础。[方法]采用石蜡切片法观察不同浓度的PP333处理后库尔勒香梨叶片解剖结构的变化,测量其叶片厚度、表皮厚度、栅栏组织厚度和海绵组织厚度,计算栅海比、叶片组织结构紧密度和叶片组织结构疏松度,分析不同生长抑制剂处理后叶片组织结构的变化。[结果]与对照相比,PP333处理后,叶片厚度、栅海比和栅栏组织厚度显著增加。PP333处理对叶片表皮的影响不明显。2 500mg/L PP333处理使叶片厚度和栅栏组织厚度增加最多。1 500 mg/L PP333处理后,叶片组织结构紧密度最高。生长抑制剂处理后,叶片海绵组织和叶片组织结构疏松度降低,2 000mg/L PP333处理的SR最低。[结论 ]不同浓度生长抑制剂处理均增大了叶片厚度、海绵组织厚度、叶片组织结构紧密度和栅海比,减小了海绵组织厚度和叶片组织结构疏松度。喷施1 500 mg/L PP333可应用于库尔勒香梨的栽培。
[Objective] The research aimed to discuss the effect of different concentrations of PP333 on the tissue structure of Korla fragrant pear leaves, and lay a foundation for the regulation of Korla fragrant pear cultivation. [Method] The changes of the leaf anatomical structure of Korla fragrant pear after different concentrations of PP333 treatment were observed by paraffin section method. The leaf thickness, epidermis thickness, palisade tissue thickness and sponge tissue thickness were measured. The leaf structure was loose, and the changes of the leaf tissue structure after different growth inhibitors were analyzed. [Result] After treatment with PP333, the thickness of leaf, the ratio of grid sea to sea wall and the thickness of palisade tissue increased significantly compared with the control. The effect of PP333 treatment on leaf epidermis was not obvious. 2 500mg / L PP333 treatment increased leaf thickness and palisade tissue thickness most. After 1 500 mg / L PP333 treatment, the leaf tissue structure was the most compact. After growth inhibitor treatment, the spongy structure of leaf spongy tissue and leaf tissue decreased, and the SR of 2 000 mg / L PP333 was the lowest. [Conclusion] The leaf thickness, sponge tissue thickness, leaf tissue structure density and grate ratio were increased by different concentrations of growth inhibitor, and the thickness of sponge tissue and the porosity of leaf tissue were decreased. Spraying 1 500 mg / L PP333 can be applied to the cultivation of Korla fragrant pear.