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利用吸收光谱和三维荧光光谱-平行因子分析(EEM-PARAFAC)方法,研究了海洋中常见的四种硅藻、两种甲藻藻液的CDOM的光吸收性质和三维荧光特性。吸收光谱测定结果表明在六种藻类生长过程中,旋链角毛藻、三角褐指藻、小新月菱形藻和盐生舟行藻四种硅藻以及东海原甲藻和裸甲藻两种甲藻的α(355)分别增加了64.8%,242.3%,535.1%,903.2%,836.0%和196.4%。表征CDOM分子量和类腐殖质组分比例的Sg呈下降趋势,分别降低了8.7%,34.6%,39.4%,53.1%,46.7%和35.7%。在三维荧光光谱测定中检测出小新月菱形藻和盐生舟行藻两种硅藻藻滤液的CDOM包括三种类腐殖质组分和一种类蛋白质组分:C1(Ex/Em=350(260)nm/450nm),C2(Ex/Em=260(430)nm/525nm),C3(Ex/Em=325nm/400nm)和C4(Ex/Em=275nm/325nm)。随着藻类的生长,小新月菱形藻和盐生舟行藻藻滤液的CDOM荧光强度分别增加了8.68,24.9,7.19,39.8倍和2.64,0.07,4.39,12.4倍,经过相关性分析表明各组分的荧光强度与α(355)和Sg之间均表现为良好的相关性。综上研究结果表明不论是甲藻还是硅藻,在生长过程中藻类内源所产生的CDOM的含量及分子量均表现为上升趋势,且硅藻类相比甲藻增长变化更为明显。在CDOM的组成中,类腐殖质成分随藻类生长所占比重同步增大,类蛋白质组分增长缓慢。另外通过该研究可以发现不同种类的藻所产生的CDOM的吸收光谱有明显差异,由三维荧光光谱得到的不同荧光组分强度也因藻种不同而不同,说明不同藻种在天然海水中对CDOM的贡献有很大区别。
The light absorption properties and three-dimensional fluorescence properties of CDOM from four kinds of diatoms and two kinds of dinoflagellate were studied by absorption spectroscopy and three-dimensional fluorescence spectroscopy-parallel factor analysis (EEM-PARAFAC). Absorption spectrometry results showed that during the growth of six species of algae, four kinds of diatoms, Chlorella spinosana, Phaeodactylum tricornutum, The α (355) of algae increased by 64.8%, 242.3%, 535.1%, 903.2%, 836.0% and 196.4% respectively. The Sg, which characterizes the molecular weight of CDOM and the proportion of humus-like components, showed a downward trend, decreasing by 8.7%, 34.6%, 39.4%, 53.1%, 46.7% and 35.7%, respectively. CDOM of two diatom filtrates detected in the three-dimensional fluorescence spectrometry was detected as Cl- and Halo-like proteins: C1 (Ex / Em = 350 (260) nm / 450nm), C2 (Ex / Em = 260 (430) nm / 525nm), C3 (Ex / Em = 325nm / 400nm) and C4 (Ex / Em = 275nm / 325nm). With the growth of algae, the CDOM fluorescence intensity of Pleurotus nebrodensis increased by 8.68, 24.9, 7.19, 39.8 and 2.64, 0.07, 4.39, and 12.4 fold, respectively. After correlation analysis, The fluorescence intensity of the fractions showed a good correlation with both α (355) and Sg. In conclusion, both the content and the molecular weight of CDOM produced by endogenous algae in the growth process are upward trend, and the diatoms are more obvious than the dinoflagellate growth. In the composition of CDOM, the proportion of humus-like components increases with the proportion of algae growth, protein-like components grow slowly. In addition, the absorption spectra of CDOM produced by different kinds of algae are obviously different. The intensity of different fluorescence components obtained by three-dimensional fluorescence spectroscopy is also different due to different algae species. The contribution is very different.