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采用高效液相色谱法测定了萨拉沙星。色谱柱为μ－BondapakTMC18柱（3．9mm×300mm），流动相为V（乙腈）：V（甲醇）：V（2mmol／L磷酸，用三乙胺调pH3．5）＝30：5：65，用二极管阵列检测器检测，检测波长278nm，得到了满意的分离效果。
Sarafloxacin was determined by high performance liquid chromatography. The column was μ-Bondapak TMMC18 column (3.9 mm × 300 mm) with a mobile phase of V (acetonitrile): V (methanol): V (2 mmol / L phosphoric acid, pH 3.5 with triethylamine) = 30: , With a diode array detector detection, detection wavelength 278nm, satisfactory separation results.