电离辐射对小鼠派伊氏板细胞凋亡的影响

• 作 者: 刘佳梅 陈东 刘树铮
• 机 构: 白求恩医科大学组胚教研室,白求恩医科大学卫生部放射生物学重点实验室
• 出 处: 辐射研究与辐射工艺学报
• 发表日期: 1999年02期
• 关键词: 电离辐射 派伊氏板 细胞凋亡 原位末端标记

采用原位末端标记（ＴＵＮＥＬ）法研究不同剂量Ｘ射线全身照射后派伊氏板细胞凋亡的时间效应及剂量效应关系。结果表明：２Ｇｙ全身照射后派伊氏板ＴＵＮＥＬ阳性细胞数增加；００５Ｇｙ、００７５Ｇｙ全身照射后派伊氏板ＴＵＮＥＬ阳性细胞数减少。提示２Ｇｙ全身照射促进细胞凋亡，而低剂量电离辐射抑制细胞凋亡。

TUNEL method was used to study the time-effect and dose-effect relationship of apoptosis induced by X-rays after the whole body irradiation. The results showed that the number of TUNEL-positive cells in the senti’s plate increased after 2Gy whole body irradiation, while the number of TUNEL positive cells in the senti’s plate decreased after 005Gy and 075Gy whole body irradiation. Prompted 2Gy whole body irradiation to promote apoptosis, while low-dose ionizing radiation inhibition of apoptosis.