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目的:探讨黑蒜水提液对人肝癌SMMC 7721细胞及小鼠肝癌Hep.A22移植性实体瘤生长的作用。方法:体外培养SMMC 7721细胞,观察黑蒜水提液干预后对细胞增殖的影响;将昆明小鼠移植H22肝癌细胞后,分阴性对照组,环磷酰胺组,黑蒜水提液低、中、高剂量组,联合加热治疗组6个组,计算黑蒜水提液对肝癌实体瘤的抑瘤率、胸腺指数和脾指数;对小鼠血清细胞因子IL-2含量测定。结果:黑蒜水提液对肝癌肿瘤均有明显的抑制生长作用,抑瘤有效率可大于40%。结论:通过确定黑蒜水提液作为纯中药抗肿瘤药物的抑瘤作用,为临床应用提供实验依据。
Objective: To investigate the effect of aqueous extract of black garlic on the growth of human hepatoma SMMC 7721 cells and mouse hepatoma Hep.A22 transplanted solid tumors. Methods: SMMC 7721 cells were cultured in vitro and the effect of aqueous extract of black garlic on cell proliferation was observed. After transplanted H22 hepatoma cells in Kunming mice, the negative control group, cyclophosphamide group, , High dose group and combined heat treatment group. The inhibitory rate, thymus index and spleen index of black garlic aqueous extract on solid tumor of liver cancer were calculated. The level of serum cytokine IL-2 in mice was determined. Results: The aqueous extract of black garlic had significant inhibitory effect on the growth of hepatocellular carcinoma. The effective rate of antitumor could be more than 40%. Conclusion: The anti-tumor effect of the aqueous extract of black garlic as a pure Chinese medicine antitumor drug can provide experimental basis for clinical application.