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采用胃粘膜体外培养技术和免疫印迹技术研究了５１例慢性胃炎和消化性溃疡患者胃粘膜局部抗幽门螺杆菌（Ｈｐ）抗体ＩｇＡ，同时对其中１０例Ｈｐ阳性患者的血清ＩｇＡ作了分析。结果表明：４０例Ｈｐ阳性患者除１例外，其粘膜抗ＨｐＩｇＡ抗体均为阳性；１１例Ｈｐ阴性患者中，２例胃粘膜局部ＩｇＡ阳性，９例为阴性。该方法与常规方法检测Ｈｐ的符合率为９２．２％。粘膜局部ＩｇＡ识别５条左右条带，且识别条带具多样性。１０例Ｈｐ阳性且局部ＩｇＡ阳性患者的血清ＩｇＡ亦为阳性，但血清ＩｇＡ的识别条带较局部ＩｇＡ少，且染色浅，说明抗ＨｇＩｇＡ抗体以局部为主，该局部ＩｇＡ的作用值得进一步研究。
Gastric mucosa local anti-Helicobacter pylori (Hp) IgA was assayed in 51 chronic gastritis and peptic ulcer patients by gastric mucosa in vitro culture and Western blotting, and serum IgA was analyzed in 10 Hp positive patients. The results showed that the anti-HpIgA antibody was positive in all the 40 Hp-positive patients except for 1 patient. Of 11 Hp-negative patients, 2 had local IgA positive in gastric mucosa, and 9 were negative. The coincidence rate of this method with the conventional method for detecting Hp was 92.2%. Local mucosal IgA recognition about 5 bands, and identification of bands with diversity. Serum IgA was also positive in 10 cases of Hp positive and local IgA positive patients. However, the serum IgA recognition band was less than that of local IgA and was stained lightly, indicating that the local anti-HgIgA antibody was localized and the role of this local IgA deserved further study.