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目的:探讨黄连提取物对耐药金黄色葡萄球菌的耐药抑制机理。方法:采用荧光分光光度法、SDS-PAGE电泳法和半自动生化仪器分别测定了黄连提取物作用前后耐药性金黄色葡萄球菌体内的药物蓄积量、细菌蛋白电泳图谱及细菌胞外某些酶活性。结果:黄连提取物处理后菌体内诺氟沙星蓄积浓度显著高于空白对照组(P<0.01);耐药金黄色葡萄球菌在35～38 kDa分子量范围内的某些蛋白质恢复表达;黄连提取物对细菌胞外酶活性无影响(P>0.05)。结论:黄连提取物对耐药金葡菌的耐药抑制作用是多种化学成分、多靶位共同作用的结果,但确切的分子水平机理还有待进一步的深入研究。
Objective: To investigate the mechanism of drug resistance of Coptis chinensis to drug-resistant Staphylococcus aureus. Methods: The drug accumulation, bacterial protein electrophoresis pattern and some extracellular enzyme activity of bacteria were detected by fluorescence spectrophotometry, SDS-PAGE electrophoresis and semi-automatic biochemical analyzer before and after the action of Coptis chinensis extract . Results: The concentration of norfloxacin in the bacteria treated with Coptis chinensis extract was significantly higher than that in the blank control group (P <0.01). Some proteins in the resistant Staphylococcus aureus were recovered in the 35-38 kDa molecular weight range. There was no effect on extracellular enzyme activity of bacteria (P> 0.05). CONCLUSION: The inhibitory effect of Coptis chinensis extract on drug-resistant Staphylococcus aureus is a result of multiple chemical components and multi-target interactions. However, the exact molecular level mechanism remains to be further studied.