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论文选取广州市天河区两家菜市场、一家海鲜市场和三家超市的48份水产品为研究对象,提取了三种不同水产品:虾、鱼和贝类的总DNA,采用实时荧光定量PCR(Quantitative Real-time PCR)法,对市售水产品中6类抗生素抗性基因(Antibiotic Resistance Genes,ARGs):tet A、tet B、tet M、sulⅡ、flo R、aph A-1、aad A、erm B、cml A和Ⅰ类整合子5’端整合酶基因int I1、3’端磺胺耐药基因sulⅠ、季铵盐化合物及溴化乙锭的耐药基因qac EΔ1进行定量。研究显示:6类ARGs及Ⅰ类整合子基因在水产品中均有检出,基因拷贝数大小从10~2~10~8跨越七个数量级,最小值为1.86×10~2 copies/g,最大值为8.98×10~8 copies/g。三种水产品虾、鱼、贝类中,虾类的ARGs含量最高,贝类最低;三类采样地点中,菜市场来源的水产品中ARGs含量最高,海鲜市场来源的次之,超市来源的最低。本研究为进一步探索ARGs和可移动耐药元件整合子介导的细菌耐药性传播而引起的食源性疾病机理奠定了基础。
In this paper, 48 aquatic products from two vegetable markets, one seafood market and three supermarkets in Tianhe District of Guangzhou were selected as research objects. Total DNA of three different aquatic products, namely shrimp, fish and shellfish, were extracted and analyzed by real-time fluorescence quantitative PCR Antibody resistance genes (ARGs): tet A, tet B, tet M, sul II, flo R, aph A-1, aad A, erm B, cml A and class Ⅰ integron 5 ’end integrase gene int I1 3’ terminal sulfa drug resistance gene sul Ⅰ, quaternary ammonium compounds and ethidium bromide resistance gene qac EΔ1 were quantified. The results showed that six kinds of ARGs and class Ⅰ integron genes were detected in aquatic products. The gene copy number spanned seven orders of magnitude from 10 ~ 2 ~ 10 ~ 8 with a minimum of 1.86 × 10 ~ 2 copies / g, The maximum value is 8.98 × 10 ~ 8 copies / g. Among the three kinds of aquatic products, shrimp, fish and shellfish had the highest ARGs content and the lowest shellfish content. Among the three sampling sites, the ARGs content of aquatic products originating from the vegetable market was the highest, followed by the seafood market source, lowest. This study laid the foundation for further exploration of the food-borne disease mechanism caused by the integration of ARGs and transfectants with integron-mediated resistance to bacteria.