论文部分内容阅读
背景:自从发现活化素可促进鸡视网膜神经细胞存活以来,活化素在神经系统的研究受到关注。近年发现,缺血、缺氧等多种脑损伤模型的海马组织活化素βAmRNA表达上调,但癫痫活动后活化素βAmRNA表达的变化有待研究。目的:观察小鼠毛果芸香碱(pilocarpine,PC)致惊后不同时间海马活化素βAmRNA的表达,探讨其作用机制。设计:以实验动物为研究对象,随机对照的实验研究。单位:一所大学医院的神经内科和一所大学神经病学研究所。材料:实验于2001-11/2002-07在复旦大学附属华山医院神经病学研究所及上海医学院病理室完成。选择8～10周龄健康雄性C57BL/6小鼠168只,体质量20～25g,由中国科学院上海实验动物中心提供。干预:实验组小鼠腹腔注射PC350mg/kg(10g/L),并于注射PC前30min皮下注射东莨菪碱1mg/kg,以拮抗其外周胆碱能反应。注射PC后呈连续的肌阵挛或全身强直阵挛发作并持续1h为癫痫持续状态(statusepilepticus,SE)模型鼠。成模后即刻腹腔注射安定(4mg/kg)终止发作,未成模鼠(NSE)于注射PC1.5h后亦注射相同剂量的安定。对照组小鼠用生理盐水代替毛果芸香碱腹腔注射,余同实验组。SE鼠、NSE鼠及对照组鼠按致模后时间随机分为0,1,3,6,24,48h6小组,每小组6只(原位杂交分析中无NSE组及0h点)。主要观察指标:应用RT-PCR?
BACKGROUND: Since the discovery that activin can promote the survival of chicken retinal neurons, activin has drawn much attention in the nervous system. In recent years, it has been found that the expression of activin βA mRNA in hippocampus is increased after ischemia, hypoxia and other brain damage models, but the changes of activin βA mRNA expression after epileptic activity need to be studied. OBJECTIVE: To observe the expression of activin βA mRNA in hippocampus of mice induced by pilocarpine (PC) at different time points and to explore its mechanism. Design: Experimental animals as the research object, a randomized controlled experimental study. Unit: Department of Neurology, University Hospital and a University Neurology Institute. MATERIALS: The experiment was performed at the Institute of Neurology, Huashan Hospital Affiliated to Fudan University and the Pathology Laboratory of Shanghai Medical College from November 2001 to July 2002. 168 healthy male C57BL / 6 mice aged 8-10 weeks were selected and their body weight was 20-25g. They were provided by Shanghai Experimental Animal Center, Chinese Academy of Sciences. Intervention: The mice in the experimental group were intraperitoneally injected with PC350mg / kg (10g / L) and scopolamine 1mg / kg subcutaneously 30min prior to PC injection to antagonize the peripheral cholinergic reaction. After injection of PC was continuous myoclonus or generalized tonic clonic seizures and sustained 1h for the status of seizure (statusepilepticus, SE) model rats. Intraperitoneal injection of diazepam (4 mg / kg) was stopped immediately after injection, and non-model rats (NSE) were injected with the same dose of diazepam 1.5 hours after injection of PC. The control group mice were injected intraperitoneally with physiological saline instead of pilocarpine, the rest of the experimental group. SE rats, NSE rats and control rats were randomly divided into 0, 1, 3, 6, 24 and 48h6 groups, with 6 mice in each group (NSE group and 0h point in in situ hybridization analysis). MAIN OUTCOME MEASURES: Application of RT-PCR?