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由乙型肝炎病毒（ＨＢＶ）引起的重症肝炎、肝硬化或肝癌而死亡者高达１００～２００万例／年。在自然宿主中仅有少数几种病毒与场症有关，ＨＢＸ蛋白致癌性较强。ＨＢＶＸ基因是ＨＢＶ４个开架阅读框中最小的一个，对应于１３７４～１８３８位核苷酸之间，编码１５４个氨基酸。该基因表达的ＨＢＸ蛋白为一种反式激活子。ＨＣＣ病人的肿瘤和肿瘤周围组织常可发现断裂Ｘ基因和完整长度Ｘ基因。本文报道利用含有同源启动子质粒ｐ４ａｌｘ和含有断裂ｘ基因质粒ｐＭＴ９Ｔ４１Ａ均建含有同源启动子的中间质粒ｐＸＰ再加上断裂Ｘ基因构建含有同源启动和断裂Ｘ基因的质粒ｐＸＰ９Ｔ４１Ａ，约４．１ｋｂ。并用该质粒与ＳＶ４０Ｌｕｃ共同转染ＮＩＨ３Ｔ３细胞，表明构建的质粒ｐＸＰ９Ｔ４１Ａ能表达ＨＢＸ蛋白，并与含异源启动子和断裂Ｘ基因的质粒ｐＭＴ９Ｔ４１Ａ的反式激活作用相类似。含有同源启动子更类似于ＨＢＶ感染者体内状况。进一步研究该质粒转基因动物中可能的病理变化与肿瘤抑制因子Ｐ５３蛋白的相互关系，与宿主细胞的整合以及细胞转化能力等，可望为ＨＣＣ的发病机理的研究提供一种有用的工具。
The number of deaths from severe hepatitis, liver cirrhosis or liver cancer caused by hepatitis B virus (HBV) is as high as 100-2 million cases / year. In the natural host only a few viruses are related to the field, HBX protein carcinogenic. The HBVX gene is the smallest of the four open reading frames of HBV4 and corresponds to nucleotides 1374 to 1838 and encodes 154 amino acids. The HBX protein expressed by this gene is a transactivator. Fracture X genes and full length X genes are commonly found in tumors and peri-tumor tissues of HCC patients. Here we report the construction of a plasmid pXP9T41A containing homologous promoter and cleavage X genes, approximately 4, using an intermediate plasmid pXP containing a homologous promoter both with a homologous promoter plasmid p4alx and a split x gene plasmid pMT9T41A plus a split X gene. 1kb. The plasmid pXP9T41A was co-transfected with NIH3T3 cells with SV40Luc, indicating that the constructed plasmid pXP9T41A can express HBX protein and is similar to the transactivation effect of the plasmid pMT9T41A containing the heterologous promoter and the split-X gene. Contains homologous promoters more similar to HBV in vivo. Further study of the possible pathological changes in the plasmid transgenic animals and the relationship between the tumor suppressor P53 protein and the host cell integration and cell transformation ability is expected to provide a useful tool for the study of the pathogenesis of HCC.