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小肠缺血再灌注的发病机制中引入了活性氧中间体(ROI,即氧自由基)的概念,因而作者推测:三碳化合物丙酮酸盐可以抑制超氧化物的产生,预防鼠小肠粘膜的再灌注损伤。材料与方法:选择6只重约250～300 g ACI 公鼠,麻醉后分离肠系膜上动脉,于小肠中段结扎约10 cm 的肠段,分别向肠腔内注入10 ml 含0.32 g 丙酮酸盐或含0.26 g 多糖的安慰剂流质,然后用血管钳阻断肠系膜上动脉45分钟,作为缺血模型,去除阻断60分钟作为再灌注模型。实验中观察:①荧光技术测定缺血再灌注时氧自由基产物浓度。②测定
The concept of reactive oxygen species (ROI), an oxygen free radical, has been introduced into the pathogenesis of ischemia-reperfusion in small intestine. Thus, the authors speculate that pyruvate, a three-carbon compound, inhibits the production of superoxide and prevents the development of murine intestinal mucosa Perfusion injury. MATERIALS AND METHODS: Six male ACI males weighing about 250-300 g were selected, the superior mesenteric artery was isolated after anesthesia, the intestine was ligated with a segment of about 10 cm in the middle part of the small intestine. 10 ml of pyruvate, Placebo fluid with 0.26 g of polysaccharide was used and then the superior mesenteric artery was blocked with a vascular clamp for 45 minutes as a model of ischemia and a block of 60 minutes was removed as a reperfusion model. Experimental observation: ① fluorescence spectrometry determination of oxygen free radical product concentration during ischemia reperfusion. ② determination