【摘 要】
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申请公布号:CN106443028A申请公布日:2017.02.22申请人:上海海洋大学摘要本发明属于化学分析检测领域,特别涉及同时测定贝类中谷胱甘肽和游离氨基酸的方法。该方法优化了样品
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申请公布号:CN106443028A申请公布日:2017.02.22申请人:上海海洋大学摘要本发明属于化学分析检测领域,特别涉及同时测定贝类中谷胱甘肽和游离氨基酸的方法。该方法优化了样品前处理程序,对分离树脂有保护作用,有利于分离,同时氨基酸提取效率更高,添加了抗氧化剂,防止谷胱甘肽被氧化,使用自制的国产缓冲液和反应液,通过改变缓冲液的p H和洗脱
Application Publication Number: CN106443028A Published Date: 2017.02.22 Applicant: Shanghai Ocean University Abstract The present invention belongs to the field of chemical analysis and detection, and particularly relates to a method for simultaneous determination of glutathione and free amino acids in shellfish. The method optimizes the sample pretreatment program, has the protective effect on the separation resin, is conducive to the separation, and the amino acid extraction efficiency is higher. The antioxidant is added to prevent the glutathione from being oxidized. The homemade domestic buffer and the reaction liquid are used, By changing the pH of the buffer and elution
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