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目的研究海参岩藻聚糖硫酸酯(SC-FUC)对长期饮酒导致的小鼠肝脏功能损伤的改善作用。方法从海地瓜(Acaudina molpadioides)中制备SC-FUC。将ICR小鼠随机分为4组:正常组、模型组以及SC-FUC低、高剂量组,每组12只。各组小鼠按0.08 ml/g bw灌胃受试物,1h后再灌胃白酒,每天一次,连续8w。实验结束后分别检测血清丙氨酸氨基转移酶(ALT)和天门冬氨酸氨基转移酶(AST)活性,肝脏乙醇脱氢酶(ADH)、乙醛脱氢酶(ALDH)、谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)活性、丙二醛(MDA)、谷胱甘肽(GSH)含量以及肝脏中CYP2e1mRNA的表达量。结果 SC-FUC能显著降低小鼠血清ALT和AST活力(P<0.05),提高肝脏ADH、ALDH、GSH-Px活力(P<0.05)和GSH含量(P<0.05),降低MDA含量(P<0.05);降低肝脏中CYP2e1mRNA的表达量(P<0.05)。结论 SC-FUC对长期饮酒小鼠肝脏具有显著的保护作用。
Objective To study the effect of sea cucumber fucoidan sulfate (SC-FUC) on the improvement of hepatic dysfunction caused by long-term alcohol consumption in mice. Methods SC-FUC was prepared from Acaudina molpadioides. The ICR mice were randomly divided into 4 groups: normal group, model group and SC-FUC low and high dose group, 12 rats in each group. Mice in each group by 0.08 ml / g bw perfusion test substance, 1h after reperfusion stomach liquor once a day for 8w. After the experiment, serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activity, hepatic alcohol dehydrogenase (ADH), aldehyde dehydrogenase (ALDH), glutathione (GSH-Px), superoxide dismutase (SOD), malondialdehyde (MDA), glutathione (GSH) and the expression of CYP2e1 mRNA in the liver. Results SC-FUC could significantly decrease the activity of ALT and AST in serum (P <0.05), increase the activities of ADH, ALDH and GSH-Px in liver and increase the content of GSH (P <0.05) 0.05), and decreased the expression of CYP2e1 mRNA in the liver (P <0.05). Conclusion SC-FUC has a significant protective effect on the liver of long-term drinking mice.