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目的比较胃癌患者与正常对照组血浆中内皮细胞微颗粒(EMPs)、血管性血友病因子(vWF)水平的变化,探讨EMPs检测在胃癌中的意义。方法采用流式细胞术及酶联免疫吸附试验(ELISA)检测68例胃癌患者和20例正常对照者血浆中EMPs、vWF的水平,分析这些指标与临床特征的关系。结果胃癌患者血浆vWF及EMPs水平显著高于正常对照组(P<0.01),血浆vWF及EMPs水平Ⅲ、Ⅳ期患者高于Ⅰ、Ⅱ患者(P<0.01),有淋巴结及肝转移者高于无淋巴结及肝转移者(P<0.01),有静脉浸润者高于无静脉浸润者(P<0.01),浸润至肌层、浆膜层者高于浸润至黏膜或黏膜下层者(P<0.01)。结论血浆EMPs可准确反映内皮功能,内皮功能紊乱参与胃癌的转移过程,EMPs可作为判断胃癌的病情、预后、疗效的指标之一。
Objective To compare the changes of plasma levels of endothelial progesophageal microvessels (EMPs) and von Willebrand factor (vWF) in patients with gastric cancer and normal controls, and to explore the significance of EMPs in gastric cancer. Methods The plasma levels of EMPs and vWF in 68 gastric cancer patients and 20 normal controls were detected by flow cytometry and enzyme-linked immunosorbent assay (ELISA). The relationship between these indexes and clinical features was analyzed. Results The levels of plasma vWF and EMPs in patients with gastric cancer were significantly higher than those in normal controls (P <0.01). The levels of vWF and EMPs in patients with stage Ⅲ and Ⅳ were higher than those in patients with Ⅰ and Ⅱ (P <0.01), and those with lymph nodes and liver metastases were higher (P <0.01), those with venous infiltration were higher than those without venous infiltration (P <0.01), those infiltrated into the muscular layer and serosal layer were higher than those infiltrating into the mucosa or submucosa (P <0.01) ). Conclusions Plasma EMPs can accurately reflect endothelial function and endothelial dysfunction in the process of gastric cancer metastasis. EMPs can be used as an indicator to judge the condition, prognosis and curative effect of gastric cancer.