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目的探讨神经生长因子(nerve growth factor,NGF)致哮喘大鼠肺内病理改变及被拮抗后肺内表达的变化,为哮喘的发病机制和治疗提供理论依据。方法随机将48只SD大鼠分为:正常对照组、哮喘组和抗NGF干预组,每组均16只。取肺组织在光镜下测量支气管基底膜厚度并计数黏膜下成纤维细胞的数目,HE染色观察气道病理改变并测量呼吸道平滑肌厚度;用免疫组化法检测肺组织NGF的表达。结果哮喘组肺组织NGF表达较对照组明显增强(P<0.01);NGF的表达与呼吸道平滑肌厚度有明显相关性;哮喘组呼吸道平滑肌厚度、网状基底膜厚度、黏膜下成纤维细胞数目及NGF的表达均高于对照组和抗NGF干预组(P<0.05);抗NGF干预组显著缓解上述变化(P<0.05)。结论支气管哮喘大鼠气道组织中NGF表达显著增加,并与呼吸道平滑肌厚度等有关,同时与支气管哮喘的气道炎症有明显相关,予抗NGF干预后可以抑制气道炎症。
Objective To investigate the changes of intrapulmonary pathological changes induced by nerve growth factor (NGF) in asthmatic rats and the expression in the lung after being antagonized, and to provide a theoretical basis for the pathogenesis and treatment of asthma. Methods 48 SD rats were randomly divided into normal control group, asthma group and anti-NGF intervention group, 16 in each group. The thickness of bronchial basement membrane was measured under light microscope and the number of submucosal fibroblasts was counted. The pathological changes of airway were observed by HE staining and the thickness of airway smooth muscle was measured. The expression of NGF in lung tissue was detected by immunohistochemistry. Results The expression of NGF in lung tissue of asthma group was significantly higher than that in control group (P <0.01). The expression of NGF was significantly correlated with the thickness of respiratory smooth muscle. The thickness of airway smooth muscle, the thickness of reticular basement membrane, the number of submucosal fibroblasts and NGF (P <0.05). The anti-NGF intervention group significantly alleviated the above changes (P <0.05). Conclusions The expression of NGF in airway of bronchial asthma rats is significantly increased, which is related to the thickness of airway smooth muscle. At the same time, airway inflammation of bronchial asthma is significantly correlated. After anti-NGF intervention, airway inflammation can be suppressed.