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目的:建立高效液相色谱法测定头孢他啶的人体血药浓度。方法:色谱柱为HypersiI C_(18)(250 mm×4.6mm,10μm)柱;流动相为甲醇-0.02 mol·L~(-1)磷酸二氢钾(13.5:86.5),流速为1 ml·min~(-1);检测波长为254 nm;柱温30℃;采用饱和硫酸铵溶液直接沉淀蛋白后进样。结果:头孢他啶血药浓度与峰面积线性关系良好;线性范围1.0～60μg·ml~(-1)(r=0.9997)。日内、日间RSD<5%:头孢他啶高、中、低浓度的平均方法回收率分别为99.2%,99.1%,98.6%,平均提取回收率分别为42.3%,42.0%,43.6%。结论:该法操作简便、准确、快速,回收率稳定,适用于头孢他啶体内动力学研究。
Objective: To establish a HPLC method for the determination of ceftazidime in human plasma. METHODS: The column was Hypersi C18 (250 mm × 4.6 mm, 10 μm). The mobile phase consisted of methanol-0.02 mol·L -1 potassium dihydrogen phosphate (13.5: 86.5) at a flow rate of 1 ml min ~ (-1). The detection wavelength was 254 nm. The column temperature was 30 ℃. The sample was precipitated directly by saturated ammonium sulfate solution. Results: The linear relationship between the concentration of ceftazidime and peak area was good. The linear range was 1.0 ~ 60μg · ml ~ (-1) (r = 0.9997). Day and day RSD <5%: The average recoveries of ceftazidime at high, medium and low concentrations were 99.2%, 99.1% and 98.6%, respectively. The average recoveries were 42.3%, 42.0% and 43.6%, respectively. Conclusion: The method is simple, accurate, rapid and stable. The method is suitable for the study of ceftazidime in vivo.