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目的探讨三氧化二砷(As2O3)联合氯化锶(89SrCl2)对人乳腺癌细胞株MCF-7细胞周期与凋亡的影响。方法采用MTT比色法检测As2O3对MCF-7细胞的增殖抑制作用,求出细胞半数抑制浓度(ID50)。选择20%ID50以下两个不同浓度的As2O3联合89Sr照射,随机设置对照组、89SrCl2照射组、As2O3处理组,As2O3+89SrCl2联合处理组(联合组),并于处理后24h采用流式细胞术检测各组细胞周期分布及凋亡。结果 As2O3能明显抑制MCF-7细胞增殖,给药24h的ID50为11.7μmol/L。细胞流式术检测结果表明,与89SrCl2照射组相比,联合组G2/M期细胞明显增多(P<0.05或0.01),早期凋亡和死亡细胞数显著增高(P<0.05)。结论 As2O3能够促进89Sr照射诱导的MCF-7细胞周期阻滞与凋亡。
Objective To investigate the effects of arsenic trioxide (As2O3) and strontium chloride (89SrCl2) on the cell cycle and apoptosis of human breast cancer cell line MCF-7. Methods The inhibitory effect of As2O3 on the proliferation of MCF-7 cells was detected by MTT colorimetric method, and the half cell inhibitory concentration (ID50) was calculated. The two different concentrations of As2O3 combined with 89Sr with 20% ID50 were selected and randomly divided into control group, 89SrCl2 irradiation group, As2O3 treatment group and As2O3 + 89SrCl2 combination treatment group (combination group), and the cells were treated with flow cytometry Cell cycle distribution and apoptosis in each group. Results As2O3 could significantly inhibit the proliferation of MCF-7 cells. The ID50 of 24h after administration was 11.7μmol / L. Cell flow cytometry results showed that compared with 89SrCl2 irradiation group, the number of G2 / M phase cells in the combined group was significantly increased (P <0.05 or 0.01), and the number of apoptotic and dead cells was significantly increased (P <0.05). Conclusion As2O3 can promote 89Sr-induced cell cycle arrest and apoptosis in MCF-7 cells.