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从多粘菌素Ｂ处理的豚鼠腹腔渗出液中，分离嗜酸性粒细胞（Ｅｏｓ）用超声粉碎仪破坏细胞，离心后收集含Ｅｏｓ成分的上清液，给豚鼠经气管插管雾化喷入。６０ｍｉｎ后豚鼠对组胺的气道反应性ＰＣ２０值显著降低（Ｐ＜０．０５）。Ｅｏｓ成分吸入处理组和正常对照组支气管肺泡灌洗液（ＢＡＬＦ）中的细胞总数、Ｅｏｓ绝对计数差异均无显著意义（Ｐ＞０．０５）。但ＢＡＬＦ细胞中上皮细胞的百分比显著增多（Ｐ＜０．００１），并且与６０ｍｉｎ时ＰＣ２０值的降低呈显著正相关（ｒ＝０．８４，Ｐ＜０．０５）。以上结果提示Ｅｏｓ可能通过其细胞成分造成气道上皮损伤诱导气道高反应性。
From polymyxin B-treated guinea pig peritoneal exudate, eosinophils (Eos) were isolated and the cells were disrupted with an ultrasonic disintegrator. After centrifugation, supernatants containing Eos components were collected and administered to guinea pigs for intubation Into. After 60 minutes, the airway responsive PC20 value of histamine was significantly decreased in guinea pigs (P <0.05). There was no significant difference in the total count of Eos and count of Eos in bronchoalveolar lavage fluid (BALF) between the Eos composition inhalation group and the normal control group (P> 0.05). However, the percentage of epithelial cells in BALF cells was significantly increased (P <0.001), and was positively correlated with the decrease of PC20 value at 60 min (r = 0.84, P <0.05). The above results suggest that Eos may induce airway hyperresponsiveness through airway epithelial damage caused by its cellular components.