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The present study was designed to characterize the polyphenols isolated from Acacia mearnsii bark crude extract(B) and fractions(B1-B7) obtained by high-speed counter-current chromatography(HSCCC) and evaluate their anti-inflammatory and carbolytic enzymes(α-glucosidase and α-amylase) inhibitory activities. Fractions B4, B5, B6, B7(total phenolics 850.3, 983.0, 843.9, and 572.5 mg·g~(-1)), respectively; proanthocyanidins 75.7, 90.5, 95.0, and 44.8 mg·g~(-1)), respectively) showed significant activities against reactive oxygen species(ROS), nitric oxide(NO) production, and expression of pro-inflammatory genes interleukin-1β(IL-1β) and inducible nitric oxide synthase(iNOS) in a lipopolysaccharide(LPS)-stimulated mouse macrophage cell line RAW 264.7. All the extracts suppressed α-glucosidase and α-amylase activities, two primary enzymes responsible for carbohydrate digestion. A. mearnsii bark samples possessed significantly stronger inhibitory effects against α-glucosidase enzyme(IC_(50) of 0.4-1.4 μg·mL~(-1)) than the pharmaceutical acarbose(IC_(50) 141.8 μg·mL~(-1))). B6 and B7(IC_(50) 17.6 and 11.7 μg·mL~(-1), respectively) exhibited α-amylase inhibitory activity as efficacious as acarbose(IC_(50) 15.4 μg·mL~(-1))). Moreover, B extract, at 25 μg·mL~(-1), significantly decreased the non-mitochondrial oxidative burst that is often associated with inflammatory response in human monocytic macrophages.
The present study was designed to characterize the polyphenols isolated from Acacia mearnsii bark crude extract (B) and fractions (B1-B7) obtained by high-speed counter-current chromatography (HSCCC) and evaluate their anti-inflammatory and carbolytic enzymes (Total phenolics 850.3, 983.0, 843.9, and 572.5 mg · g -1, respectively), respectively; and proanthocyanidins 75.7, 90.5, 95.0, and 44.8 mg · G -1 showed significant activities against reactive oxygen species (ROS), nitric oxide (NO) production, and expression of pro-inflammatory genes interleukin-1β (IL-1β) and inducible nitric oxide synthase iNOS) in a lipopolysaccharide (LPS) -stimulated mouse macrophage cell line RAW 264.7. All the extracts suppressed alpha-glucosidase and alpha-amylase activities, two primary enzymes responsible for carbohydrate digestion. A. mearnsii bark samples possessed significantly arrested inhibitory effects against alpha -glucosidase enzyme (IC 50 (50) of 0.4-1.4 μg · mL -1) than the pharmaceutical acarbose (IC 50 (141.8 μg · mL -1)). B6 and B7 μg · mL -1, respectively) showed α-amylase inhibitory activity as efficacious as acarbose (IC 50 (15.4 μg · mL -1)). Moreover, B extract at 25 μg · mL -1 -1), significantly decreased the non-mitochondrial oxidative burst that is often associated with inflammatory response in human monocytic macrophages.