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目的 评价125Ⅰ-脱氧尿嘧啶核苷(UdR)对膀胱癌细胞Sca-BER生长的辐射生物作用及其影响因素。方法将125Ⅰ UdR加入Sca-BER细胞培养基中,测量细胞摄取125Ⅰ UdR的放射性活度,用细胞克隆形成法测定125Ⅰ UdR 对Sca-BER细胞的生长抑制作用;将125Ⅰ UdR和MTX同时加入Sca-BER细胞培养基中,测量细胞摄取125ⅠUdR的放射性活度,观察MTX协同125Ⅰ UdR的生长抑制作用。结果 培养基中125Ⅰ UdR浓度与Sca-BER细胞摄取量,两者之间呈显著正相关(r=0.9999);Sca-BER细胞存活分数与125IUdR浓度,两者呈负相关(r=-0.9),半数致死剂量(LD50)为(1.17±0.27)kBq／ml。125Ⅰ UdR组细胞存活分数明显低于Na125Ⅰ组;相同浓度125Ⅰ UdR作用于Sca-BER细胞生长过程中,在24 h内细胞摄取量随作用时间的延长而增加,24 h后达最大;甲氨喋呤协同125ⅠUdR抑制Sca-BER细胞生长,使细胞摄取量提高10倍。结论125Ⅰ UdR能掺入到Sca-BER细胞中,并对其有显著辐射抑制效应,说明125Ⅰ UdR可以进一步应用于膀胱癌的放射治疗研究。
Objective To evaluate the radiobiological effects of 125 I-deoxyuridine (UdR) on the growth of Sca-BER in bladder cancer cells and its influencing factors. Methods 125I UdR was added into Sca-BER cell culture medium to measure radioactive activity of 125I UdR, and the growth inhibition of 125I UdR on Sca-BER cells was determined by cell clone formation method. 125I UdR and MTX were simultaneously added to Sca- BER cell culture medium, radioactivity of 125 IUdR was measured by cellular uptake, and the growth inhibitory effect of MTX in combination with 125 I UdR was observed. Results There was a significant positive correlation between the concentration of 125 I UdR and the uptake of Sca-BER in the medium (r = 0.9999). The survival rate of Sca-BER cells was negatively correlated with the concentration of 125 IUdR (r = -0.9) , And the LD50 was (1.17 ± 0.27) kBq / ml. The cell viability of 125I UdR group was significantly lower than that of Na125I group. With the same concentration of 125I UdR, the uptake of 125IUdR in Sca-BER cells increased with the prolongation of action time and reached the maximum at 24 h. The synergistic 125 IUdR inhibits the growth of Sca-BER cells and increases the cellular uptake by 10-fold. Conclusion 125 Ⅰ UdR can be incorporated into Sca-BER cells and has a significant effect of inhibiting radiation, indicating that 125 Ⅰ UdR can be further applied to the study of radiation therapy of bladder cancer.