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目的：探讨间日疟原虫环子孢子蛋白（ＣＳＰ）基因多态性的地理特征及其实用意义。方法：用ＤＮＡ的ＰＣＲ扩增与电泳切带纯化法，获得所研究的基因片段；用引物标记周期反应测序法，测定ＤＮＡ序列；用图解法分析比较各地ＣＳＰ基因的氨基酸序列的变化。结果：所测定的中国、菲律宾和所罗门的１５个间日疟分离株均属ＰＶ－１型，但各株有较明显的地理性变异。６个中国株与ＮＫ株相似，其中Ｃ－２与ＮＫ株完全一致，而与其他Ⅰ型株有明显区别。结论：根据ＣＳＰ基因中央重复区及重复后可变区推导的氨基酸序列的变异情况，在Ｑａｒｉｔ和Ｍａｎｎ的分组基础上，迄今已发表的所有Ⅰ型株可归纳为２族６个组。分组序列特征有可能用于不同地理株的检出和鉴定，从而在疟疾的流行病学与防治上有实用价值，并将有助于疟原虫的生物学、分类学和种系发生的研究。
Objective: To investigate the geographical characteristics of Plasmodium vivax sporozoite protein (CSP) gene polymorphism and its practical significance. Methods: The DNA fragment was amplified by PCR and purified by electrophoresis. The DNA fragment was sequenced by primer reaction and sequenced. The amino acid sequence of CSP gene was analyzed by graphic method. RESULTS: The 15 P. vivax isolates tested in China, the Philippines and Solomon Islands belonged to PV-1 type, but each strain had obvious geographical variation. Six Chinese strains were similar to NK strain, of which C-2 was completely identical with NK strain but distinct from other type I strains. CONCLUSION: Based on the variation of amino acid sequences deduced from the central repeat region and variable region after repeat of CSP gene, all type Ⅰ strains published so far can be grouped into 2 groups and 6 groups based on the grouping of Qarit and Mann. The characteristics of grouping sequences may be used for the detection and identification of different geographic strains, which have practical value in the epidemiology and prevention of malaria and will be helpful for the biology, taxonomy and germline genesis of the parasite.