论文部分内容阅读
详细研究了酒石酸（以Ｔ表示）铜（以Ｃ表示）配合物（常称的费林试剂，简写成ＴＣ）用于还原糖测定的分光光度法。结果表明，ＮａＯＨ浓度大于０．０８０ｍｏｌ·Ｌ－１时ＴＣ的最大吸收波长为６７２ｎｍ，在ＮａＯＨ浓度为０．１６ｍｏｌ·Ｌ－１碱性介质中，配合物ＴＣ的吸光度与还原糖在０．２９～１４．７２ｍｇ（六碳糖）范围内有很好的线性关系，相关系数大于０．９９８，还原糖中每个起作用的碳约消耗１．０个Ｃｕ２＋，经对棉花及部分水果样品中还原糖总量的测定，回收率在９４％～１０７％之间。该法试剂易得，性质稳定，操作方便，灵敏度较高，取样量少于５ｍｌ。
A detailed study of tartaric acid (in T) copper (C) complex (commonly known as Feilin reagent, abbreviated as TC) for the determination of reducing sugar spectrophotometry. The results showed that the maximum absorption wavelength of TC was 672 nm when the NaOH concentration was more than 0.080 mol·L-1. The absorbance of the TC of the complex was lower than that of the reducing sugar at 0.29 ~ 14.72mg (six-carbon sugar), the correlation coefficient is greater than 0.998. Each functionalized carbon in reducing sugar consumes about 1.0 Cu2 +, and in cotton and some fruit samples Determination of the total amount of reducing sugar, the recovery rate of 94% to 107%. The reagent is easy to get, stable in nature, convenient in operation and high in sensitivity. The sampling volume is less than 5ml.