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本研究应用免疫荧光组织化学三重染色技术和免疫电镜双重标记技术,对大鼠囊泡膜谷氨酸转运体 (VGluT1和VGluT2)和 5 -羟色胺(5- HT)阳性终末与三叉神经中脑核(Vme)神经元之间的联系进行了观察。在激光共聚焦显微镜下,Vme内可观察到许多磷酸激活的谷氨酰胺酶(PAG)阳性神经元,其中绝大多数为大的假单极神经元。VGluT1和VGluT2免疫阳性的神经纤维和终末广泛分布于Vme内,其中VGluT2阳性纤维和终末的分布密度高于VGluT1。在Vme内还可观察到相当数量的 5- HT阳性终末,其中部分终末同时呈VGluT2免疫阳性。一些VGluT1、VGluT2、5- HT及VGluT2 /5- HT双重标记的阳性终末与PAG阳性的Vme神经元胞体形成密切接触。在电镜下,分别用银加强的金颗粒和DAB反应产物显示VGluT1 /VGluT2和 5- HT阳性终末。在Vme内只观察到部分终末呈VGluT2和 5- HT双重免疫阳性,并且这些终末主要形成非对称性突触。以上结果提示,在口面部本体感觉信息向更高一级中枢传递过程中,谷氨酸能和 5 HT能神经终末可能通过Vme神经元对该信息进行复杂的调控。
In this study, immunofluorescence histochemical triple staining technique and immunoelectron microscopy double labeling technique were used to detect the expression of glutamate transporter (VGluT1 and VGluT2) and serotonin (5 HT) in rat middle cerebral artery Nuclear (Vme) neurons were observed. Under laser confocal microscopy, many phospho-activated glutamase (PAG) positive neurons were observed in Vme, the vast majority of which were large pseudopolar neurons. VGluT1 and VGluT2 immunoreactive nerve fibers and terminals are widely distributed in Vme, VGluT2-positive fibers and terminal distribution density higher than VGluT1. A significant number of 5-HT positive terminals were also observed in the Vme, some of which were simultaneously immunogenically positive for VGluT2. Some VGluT1, VGluT2, 5-HT, and VGluT2 / 5-HT double-labeled positive terminals are in intimate contact with PAG-positive Vme neuronal soma. Under electron microscopy, VGluT1 / VGluT2 and 5-HT positive termini were displayed with silver-enhanced gold particles and DAB reaction products, respectively. Only partial terminals were observed as positive for VGluT2 and 5-HT in Vme, and these terminals mainly formed asymmetric synapses. The above results suggest that glutamate and 5 HT neurotactic terminals may complexly regulate this information through the Vme neurons in the process of transmitting sensory information to the higher level in the oral and facial ontology.