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目的内皮抑素(endostatin)是强烈的抗血管再生因子。本文探讨大脑中动脉闭塞 (middle cerebral artery occlusion, MCAO)致局灶性脑缺血后脑组织内皮抑素蛋白及mRNA基因表达的变化,同时检测缺血脑组织血管内皮生长因子(vascular endothelial growth factor,VEGF)的含量。方法24只新西兰白兔随机分为正常对照组(n=5)、假手术组(n=4)、缺血2小时组(n=5)、缺血24小时组 (n=5)及缺血48小时组(n=5)共5组。酶联免疫吸附试验测定VEGF含量,免疫组化分析内皮抑素蛋白变化,原位杂交检测内皮抑素mRNA表达。结果与对照组相比,MCAO局灶性脑缺血后内皮抑素蛋白和mRNA表达均明显增加,至少分别增加了50%(P<0.01)和70%(P<0.05),同时缺血脑组织VEGF含量也明显增加,至少增加了270%。结论缺血导致脑组织内皮抑素表达增加,且内皮抑素的增加与缺血后脑组织VEGF变化无相关性,但可能抑制脑缺血后的血管再生,从而加重脑缺血损伤。
Purpose Endostatin is a potent anti-angiogenesis factor. This article discusses the changes of endothelin gene and mRNA expression in cerebral tissue after focal cerebral ischemia induced by middle cerebral artery occlusion (MCAO), and to detect the expression of vascular endothelial growth factor VEGF) content. Methods Twenty-four New Zealand white rabbits were randomly divided into normal control group (n = 5), sham operation group (n = 4), ischemia 2 hours group (n = 5), ischemia 24 hours group Blood 48 hours group (n = 5) a total of 5 groups. The content of VEGF was determined by enzyme - linked immunosorbent assay, the change of endostatin protein by immunohistochemistry and the expression of endostatin mRNA by in situ hybridization. Results Compared with the control group, the expression of endostatin protein and mRNA increased significantly after focal cerebral ischemia in MCAO by at least 50% (P <0.01) and 70% (P <0.05), respectively. Meanwhile, the ischemic brain Tissue VEGF content also increased significantly, at least increased by 270%. Conclusion Ischemia leads to the increase of endostatin expression in brain tissue, and the increase of endostatin has no correlation with the change of VEGF in ischemic brain tissue. However, it may inhibit the angiogenesis after cerebral ischemia and further aggravate cerebral ischemia.