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目的:研究千根草水提取物对小鼠D-半乳糖胺盐酸盐(D-GalN)致急性肝损伤的保护作用。方法:将实验小鼠随机分成6组:正常对照组,模型组,联苯双酯组(150mg/kg/d),千根草水提物低(5g/kg/d)、中(10 g/kg/d)、高剂量组(20g/kg/d),并设正常对照组。连续ig给药7d,末次灌胃1h后,正常对照组小鼠腹腔注射生理盐水,各给药组及模型组小鼠腹腔注射D-GalN(800mg/kg),造成化学性肝损伤模型,16h后称重取血取材。观测肝脏系数;测定血清谷丙转氨酶(ALT)、谷草转氨酶(AST)、碱性磷酸酶(ALP)活性和总蛋白(TP)、白蛋白(ALB)、球蛋白(GLB)、白球比(A/G)含量。结果:与模型组相比,千根草水提物各剂量组及阳性对照组均能增加小鼠体重,并能降低肝脏系数,同时能降低小鼠血清中ALT、AST、ALP水平和含量(P<0.01或P<0.05),提高小鼠血清中TP、ALB、GLB、A/G(P<0.01或P<0.05)。结论:千根草水提取物对D-GalN致急性肝损伤的小鼠有保护作用,对肝损伤的防治有良好的潜在应用价值。
Objective: To study the protective effect of 1000 roots aqueous extract on acute liver injury induced by D-galactosamine hydrochloride (D-GalN) in mice. Methods: The experimental mice were randomly divided into 6 groups: normal control group, model group, bifendate group (150mg / kg / d), Aqaba extract low (5g / / kg / d), high dose group (20g / kg / d) and normal control group. The rats in the normal control group were intraperitoneally injected with normal saline for 7 days after the last ig administration, and the mice in each administration group and the model group were intraperitoneally injected with D-GalN (800 mg / kg), causing chemical liver injury model, 16 h After weighing blood drawn. The liver coefficient was measured. The activities of serum ALT, AST, ALP, total albumin (ALB), globulin (GLB) and white ball ratio / G) content. Results: Compared with the model group, each dose group and the positive control group could increase the body weight of mice and decrease the hepatic coefficient, at the same time, it could reduce the level and content of ALT, AST and ALP P <0.01 or P <0.05), and increased TP, ALB, GLB, A / G in serum (P <0.01 or P <0.05). Conclusion: The water extract of 1000 roots has the protective effect on D-GalN-induced acute liver injury in mice and has a good potential value for the prevention and treatment of liver injury.