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对甘薯近缘野生种 I. littoralis Blune 愈伤组织植株再生进行研究。将叶片、叶柄外植 体培养在添加0 .02 ~0 .05 m g/ L2 ,4 - D 和0 .5 ~2 .0 m g/ L 激动素( K T) 的 M S 培养基上,诱导愈伤组织。将形成的愈伤组织转移到 M S 基本培养基上3 ~4 周后,观 察到植株再生。叶片、叶柄愈伤组织再生 植株频率分别达55 .5 % 和50 .0 % 。
Sweet potato relatives wild species I. The littoralis Blune callus plant regeneration was studied. Leaves and petiole explants were cultured at 0. 02 ~ 0. 05 m g / L2, 4 - D and 0. 5 ~ 2. 0 m g / L kinetin (K T) of M S medium, callus induction. Three to four weeks after the callus formation is transferred to M S minimal medium, plant regeneration is observed. Leaf, petiole callus regeneration plants frequency of 55 respectively. 5% and 50. 0%.