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染色体异常伴有各种类型的人类白血病和淋巴瘤的特征,正成为鉴别诊断和治疗这些疾病的可能方法。然而,通常骨髓标本核型显带的低质量,以及需要长时间的分析方法,防碍了这些疾病在临床上应用核型分析。这些分析技术常常需要24～48小时的培养时间,以获得有丝分裂相;Q显带—需要摄影核型进行分析;胰酶G显带—也要耗费许多时间,通常亦不能提供高质量带型。为了使染色体分析有助于血液病的诊断和治疗,必须改善骨髓染色体标本制备的质量和速度。作者提出了一个骨髓抽出2小时后即可进行分析,并产生高质量带型的骨髓中期染色体显带方法。这个方法的主要特点是直接处置骨髓抽出液,开始用秋水仙胺温育,然后用胰酶低渗处置,应用Wright’s显带染色技术的改进方法,在显微镜下及时地进
Chromosomal abnormalities, accompanied by various types of human leukemias and lymphomas, are becoming possible methods of differential diagnosis and treatment of these diseases. However, the low quality of the karyotyping of myeloid specimens and the long-term analytical methods that hinder the clinical application of karyotyping in these diseases are of the opinion. These analytical techniques often require 24 to 48 hours of culture time to obtain the mitotic phase; Q banding - requires photographic karyotype analysis; trypan G-banding - also takes a lot of time and usually does not provide high-quality banding. In order for chromosomal analysis to aid in the diagnosis and treatment of blood diseases, the quality and speed of the preparation of bone marrow astrocytes must be improved. The authors propose that a bone marrow can be analyzed 2 hours after extraction and produce a high-quality banding of the metaphase chromosome in the bone marrow. The main feature of this method is the direct treatment of bone marrow extract, began with colchicine incubation, and then treated with pancreatic hypotonic treatment, the use of Wright’s banding staining method of improvement, in a timely manner under the microscope