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人参是著名的补益类中草药,具有广泛的药理作用。而人参皂甙是人参的主要药用有效物质。国内已有人从免疫学的角度研究人参花总皂甙,但尚未见到人参花总皂甙影响NK活性的报导。本实验首先就~(125)IUdR释放法的方法学作了一些探讨,然后观察了人参花总皂甙在体外对小鼠脾细胞NK活性的影响。材料与方法一、材料: (1)人参皂甙(ginsenoside缩写GS):本校化学教研室制备、鉴定后供给。(2)YAC—1细胞:医科院基础研究所免疫室惠赠。(3)IMDM培养基:美国Grand Island Biological Company生产。(4)~(125)IUdR(~(125)I脱氧尿嘧啶核苷):
Ginseng is a famous tonic herbal medicine with extensive pharmacological effects. Ginsenosides are the main medicinal substances of ginseng. In China, there have been studies on ginsenosides from the perspective of immunology, but no report has been made on the effects of ginsenosides on NK activity. In this experiment, we first discussed the method of ~(125)IUdR release method, and then observed the effect of total saponins of Panax ginseng on the splenocyte NK activity in mice. Materials and Methods I. Materials: (1) Ginsenoside (GS GS): Prepared and identified by the Chemistry Teaching and Research Section of the school. (2) YAC-1 cells: A gift from the Immunology Institute of the Basic Research Institute of the Chinese Academy of Medical Sciences. (3) IMDM medium: produced by Grand Island Biological Company, USA. (4) ~(125)IUdR(~(125)I deoxyuridine):