论文部分内容阅读
目的 :调查宁化林区动物感染斑点热群立克次体 (SFGR)情况。方法 :鼠类、野兔、野猪、麂、狐狸、狗、牛血清学检测 ,采用微量室温补体结合法 (mCF) ;病原分离 :用鸡胚卵黄囊感染法分离SFGR ,并用mCF、PCR/RFLP技术对分离株进行鉴定。结果 :鼠类、野兔、野猪、麂、狐狸、狗等动物存在SFGR感染 ,其抗体阳性率分别为 11.2 0 %、5 .88%、5 0 .0 0 %、2 0 .0 0 %、5 5 .5 6%、6.67% ,牛血清中未检出SFGR抗体。查出在上述动物体表寄生的蜱类 6属 13种 ,鉴定分类 5 0 0 0余只 ,先后从野兔、麂体表寄生的越原血蜱中分离出 2株SFGR病原体。结论 :从宿主动物、媒介蜱、病原学上进一步证实福建宁化存在斑点热群立克次体自然疫源地。
Objective: To investigate the situation of spotted fever group Rickettsia (SFGR) in Ninghua forest area. Methods: Murine, rabbits, wild boar, muntjac, foxes, dogs and bovine serological tests were performed with trace amount of room temperature complement fixation (mCF). Pathogen isolation: SFGR was isolated from chick embryo yolk sac by mCF and PCR / RFLP Isolates were identified. Results: The positive rate of SFGR infection in mice, rabbits, wild boar, muntjac, foxes and dogs was 11.2 0%, 5.88%, 50.0%, 20.0%, 5 5.56% and 6.67%, respectively. No SFGR antibodies were detected in bovine serum. Among the 13 species of 6 genera and 6 species of ticks parasitized on the body surface of the above animals, more than 500 species of the ticks were identified. Conclusion: From the host animals, ticks ticks, etiology further confirmed that Fujian Ninghua spotted fever group Rickettsia natural foci.