论文部分内容阅读
利用爪哇稻NS2098与籼稻桂农占杂交并自交获得的F2群体,对水稻落粒性进行了遗传分析和基因定位。结果表明,难落粒性状受1对显性基因Sh(t)控制。利用分子标记将sh(t)定位在第1号染色体的SSR标记RM11869、RM3825、RM11875、RM8278、RM315、RM11893和RM1387附近,与它们之间的遗传距离分别是1.65,1.59,1.46,1.65,1.72,1.72和4.13 cM,与落粒性基因qSH1的1个SNP临近。序列分析表明,NS2098在该SNP位点为碱基T,而桂农占为碱基G,与qSH1基因的报道结果一致,推测Sh(t)与qSH1可能是同一基因。
Genetic analysis and gene mapping were performed on the shattering of rice using F2 population derived from a hybrid between Java2020 and indica Guinong. The results showed that the trapping resistance was controlled by one pair of dominant genes Sh (t). The molecular marker was used to map sh (t) in the vicinity of the SSR markers RM11869, RM3825, RM11875, RM8278, RM315, RM11893 and RM1387 on chromosome 1. The genetic distances among them were 1.65,1.59,1.46,1.65,1.72 , 1.72 and 4.13 cM, respectively, and 1 SNP of the shatter gene qSH1. Sequence analysis showed that NS2098 was a base T at this SNP site, while Guinong accounted for base G, which was consistent with the reported result of qSH1 gene. It is speculated that Sh (t) and qSH1 may be the same gene.