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用于基因定量检测的定量PCR荧光检测系统多采用共聚焦式光学模块实现荧光信号的激发与传输,由于系统中光学元件相对较多,影响了荧光检测系统的检测精度。为提高基因定量检测的准确度和精度,对共聚焦式荧光检测模块进行了改进与再设计。通过采用Y型光纤和高精度PMT构建出Y光纤型荧光检测系统,不仅简化了光学系统的结构,还有效降低了光信号在传播过程中的损耗。仿真实验表明采用光纤型荧光检测系统有效提高了系统荧光检测结果的精度,这将大大改善系统基因定量结果的准确度,从而保证定量PCR系统基因定量分析的可靠性。
The quantitative PCR fluorescence detection system used for gene quantitative detection mostly adopts the confocal optical module to realize the excitation and transmission of fluorescence signals. Since the number of optical elements in the system is relatively large, the detection accuracy of the fluorescence detection system is affected. In order to improve the accuracy and accuracy of gene quantitative detection, the confocal fluorescence detection module was improved and redesigned. By using Y-fiber and high-precision PMT to construct the Y-fiber fluorescence detection system, not only the structure of the optical system is simplified, but also the loss of the optical signal in the propagation process is effectively reduced. The simulation results show that the optical fiber fluorescence detection system can effectively improve the accuracy of the system fluorescence detection results, which will greatly improve the accuracy of the system gene quantitative results and ensure the quantitative analysis of the quantitative PCR system.