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目的观察大黄酚固体分散体对镉染毒大鼠氧化损伤的影响。方法以聚乙二醇6 000为载体制备大黄酚固体分散体,差示扫描量热法(DSC)和X射线衍射法对固体分散体进行分析。SD大鼠40只,雌雄各半,随机分为5组,分别为正常对照组、镉染毒组、镉加大黄酚组、镉加大黄酚固体分散体低剂量组和镉加大黄酚固体分散体高剂量组。正常对照组灌胃纯净水,其余4组给予20 mg/kg氯化镉隔日灌胃染毒,共染毒4次,染毒6 h后给予大黄酚或大黄酚固体分散体干预。末次给药后,测定大鼠血清、脑和肾中超氧化物歧化酶(SOD)活力及丙二醛(MDA)水平,测定大鼠血清谷胱甘肽过氧化物酶(GSH-Px)、丙氨酸转氨酶(ALT)和天冬氨酸转氨酶(AST)活力。结果 DSC和X射线衍射分析表明,大黄酚以无定形形式存在于固体分散体中。大黄酚固体分散体可降低镉染毒大鼠血清、脑组织和肾中MDA水平(P<0.05),增加血清、脑组织和肾中SOD和GSH-Px活力(P<0.05),降低血清中ALT、AST活力(P<0.05) 大黄酚固体分散体可降低镉对大鼠造成的肝损伤、脑损伤和氧化损伤。
Objective To observe the effect of chrysophanol solid dispersion on oxidative damage in cadmium-exposed rats. Methods Solid dispersions of chrysophanol were prepared by using polyethylene glycol 6000 as a carrier. The solid dispersions were analyzed by differential scanning calorimetry (DSC) and X-ray diffraction. Forty SD rats were randomly divided into 5 groups: normal control group, cadmium group, chondroitin plus cadmium group and low dose chondroitin plus cadmium plus chondroitin solid dispersion Body high dose group. The normal control group was infused with pure water, and the other four groups were given intragastric administration of 20 mg / kg cadmium chloride every other day for 4 times. After 6 hours of exposure, chrysophanol or chrysophanol solid dispersion was given for intervention. After the last administration, the activity of superoxide dismutase (SOD) and the content of malondialdehyde (MDA) in serum, brain and kidney of rats were measured, and the levels of serum glutathione peroxidase (GSH-Px) Aminotransferase (ALT) and aspartate aminotransferase (AST) activity. Results DSC and X-ray diffraction analysis showed that chrysophanol was present in amorphous form in a solid dispersion. Chrysophanol solid dispersions could reduce the level of MDA in serum, brain and kidney (P <0.05), increase the activities of SOD and GSH-Px in serum, brain and kidney (P <0.05) ALT, AST vitality (P <0.05) Chrysophanol solid dispersion can reduce liver injury, brain injury and oxidative damage caused by cadmium in rats.