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目的分析吉林省敦化市流行的肠道病毒71型分离株的基因特征。方法对2009-2011年敦化市手足口病来源的3株EV71分离株进行逆转录-聚合酶链反应扩增VP1基因编码区后进行序列测定,并使用Bioedit和Maga4生物信息学软件进行基因特征分析。结果敦化市3株EV71流行株均为C4a基因亚型,3株EV71分离株之间核苷酸同源性为98.6%~98.8%;与吉林省和龙市的流行株核苷酸同源性为98.8%~99.8%,与吉林省其他地区的2株EV71代表株的核苷酸同源性为97.6%~99.3%,与安徽省阜阳市2008年代表株的核苷酸同源性为98.3%~99.3%。敦化市3株EV71分离株之间、与2008年阜阳株以及吉林省2009-2011年3株代表株的氨基酸同源性均达到100%。结论敦化市3株EV71分离株与同为延边州的和龙市的流行株核苷酸同源性最高,与安徽省阜阳市2008年流行株的核苷酸也具有较近的同源性。敦化市3株EV71分离株之间以及与中国C4a基因型代表株之间都具有较高的氨基酸同源性。
Objective To analyze the genetic characteristics of enterovirus 71 (Enterovirus 71) strains prevailing in Dunhua City, Jilin Province. Methods RT - PCR was used to amplify the coding region of VP1 gene in three EV71 isolates from Dunhua City in 2009-2011. The sequence of the VP1 gene was then sequenced and analyzed using Bioedit and Maga4 bioinformatics software . Results The three strains of EV71 in Dunhua City were subtypes of C4a gene. The nucleotide homology of the three strains of EV71 was 98.6% ~ 98.8%. Nucleotide homology with the strains of Helicobacter pylori Was 98.8% ~ 99.8%. The nucleotide homology between the two strains of EV71 and other strains in Jilin Province was 97.6% ~ 99.3%. The nucleotide homology was 98.3 with the representative strain of Fuyang in Anhui Province in 2008 % ~ 99.3%. The amino acid homology between the three EV71 isolates in Dunhua City and Fuyang strain in 2008 and the three strains in Jilin province from 2009 to 2011 reached 100%. Conclusion The nucleotide sequences of three EV71 isolates in Dunhua City and those in the same city in Yanbian Prefecture are the most similar. There were high amino acid homologies among the three EV71 isolates in Dunhua and the C4a genotypes in China.