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目的:建立大鼠血浆中克班宁浓度的高效液相色谱(HPLC)测定方法,探讨盐酸克班宁在大鼠体内的药动学过程。方法:按5.0mg.kg-1量大鼠尾静脉注射盐酸克班宁,在规定时间内采样,用HPLC法测定血浆中克班宁浓度,以DAS软件拟合药动学参数。结果:盐酸克班宁在大鼠体内呈开放一室模型,雌、雄大鼠体内的药动学参数经t检验差异无显著性。主要药动学参数为t1/2=(39.7±3.9)min,Cmax=(6.56±0.31)mg.L-1,CL=(0.055±0.005)L.min-1.kg-1,Vd=(2.95±0.28)L.kg-1。结论:试验初步揭示了盐酸克班宁在大鼠体内的药动学规律。盐酸克班宁在大鼠体内为超速处置类药物(t1/2<1h),分布迅速、广泛。
OBJECTIVE: To establish a high performance liquid chromatography (HPLC) method for the determination of Kebanin concentration in rat plasma and investigate the pharmacokinetics of Kebanin in rats. Methods: Ketanin was injected into tail vein of rats by 5.0mg.kg-1, and sampled within the prescribed time. The concentration of 克南宁 in plasma was determined by HPLC. The pharmacokinetic parameters were fitted by DAS software. Results: Kebaning hydrochloride was an open one-compartment model in rats. There was no significant difference in pharmacokinetic parameters between female and male rats by t-test. The main pharmacokinetic parameters were t1 / 2 = (39.7 ± 3.9) min, Cmax = 6.56 ± 0.31 mg.L-1, CL = 0.055 ± 0.005 L.min-1.kg- 2.95 ± 0.28) L.kg-1. Conclusion: The experiment initially revealed the pharmacokinetics of Kebanin in rats. Clenbuterol in rats for speeding drugs (t1 / 2 <1h), the distribution of rapid and extensive.